Detection of B 6 Vitamers in Grain Products: Experimental and Computational Studies
- PDF / 702,348 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 91 Downloads / 175 Views
Detection of B6 Vitamers in Grain Products: Experimental and Computational Studies Anna Lebiedzińska 1 & Marcin L. Marszałł 2 & Małgorzata Grembecka 1 & Jakub Czaja 1 & Piotr Szefer 1 & Jadwiga Kuta 3 & Brady D. Garabato 3 & Pawel M. Kozlowski 1,3
Received: 19 May 2017 / Accepted: 20 September 2017 / Published online: 1 October 2017 # The Author(s) 2017. This article is an open access publication
Abstract A reversed phase high-performance liquid chromatography method (HPLC) with coulometric electrochemical detection has been applied and validated for the simultaneous analysis of pyridoxamine (PM), pyridoxal (PL), and pyridoxine (PN) in cereal products. Isocratic separation was achieved using a C18 column with a mobile phase consisting of methanol-phosphate buffer (10:90) and 0.018 M trimethylamine adjusted to pH 3.55 with 85% orthophosphoric acid. The limits of detection for PM, PL, and PN were 0.28, 0.36, and 0.43 ng mL−1, respectively, with vitamins recoveries ranging from 90.4 to 98.1%. The applied method for the analysis of B6 vitamins naturally present in grain products, offers a simple and fast sample preparation without derivatization. To understand vitamer separation further, methods of computational chemistry were employed. Specifically, density functional theory (DFT) was used to determine electrostatic potentials, as well as the charges of each vitamer. A number of correlations were established between these properties and elution order.
Pawel M. Kozlowski is a visiting professor at the Department of Food Sciences, Medical University of Gdansk. * Anna Lebiedzińska [email protected] * Pawel M. Kozlowski [email protected] 1
Department of Food Sciences, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland
2
Department of Toxicology, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland
3
Department of Chemistry, University of Louisville, 2330 South Brook Street, Louisville, KY 40292, USA
Keywords Cereal products . Pyridoxal . Pyridoxine . Pyridoxamine . HPLC . Coulometric detection . Quantum-chemical calculations . Density functional theory
Introduction The B6 vitamers (Fig. 1) are a group of water-soluble forms of vitamin B6, which are chemically quite distinct compounds. Vitamin B6 represents a group of highly bioavailable 3hydroxy-2-methylpyridine derivatives that exist in food as phosphorylated and non-phosphorylated forms (Ball 2006; Eitenmiller et al. 2008). Vitamin B6 are also enzymatic cofactors required for over 140 biochemical reactions involved in amino acid biosynthesis and degradation, as well as sugar, and fatty acid metabolism (Ball 2006). Vitamin B6 is necessary in the synthesis of both serotonin and GABA neurotransmitters that control depression, pain perception, and anxiety. Most vitamin B6-dependent enzymes catalyze reactions including transamination, decarboxylation, dehydration, desulfhydration, racemization, cleavage, and synthesis, among other processes including the activation of glycine during the initial stages of heme produ
Data Loading...
