Detection of energy transfer mechanisms in nanoscopic optical rulers
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RESEARCH PAPER
Detection of energy transfer mechanisms in nanoscopic optical rulers Anh Thi Le & Minh Hoa Nguyen & T. Anh Thu Do & Minh Tan Man
Received: 22 June 2020 / Accepted: 16 October 2020 # Springer Nature B.V. 2020
Abstract Understanding the energy transfer dynamics in fluorescein-gold nanoparticle systems is crucial for future optoelectronic devices based on energy transfer. Dominant energy transfer mechanisms including the dipole-dipole (FRET) and dipole-surface energy transfer (SET) are investigated. We have compared the measured performance values with the theoretical predictions for both FRET and SET models, specifically the importance of interactions between various scales. It has been shown that the highly sensitive FRET process is limited and focused on the Förster radius range (R0), while the high efficiency of the SET over longer distances provides better resolution than the FRET
A. T. Le Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam A. T. Le : M. T. Man Faculty of Natural Sciences, Duy Tan University, Da Nang 550000, Vietnam M. H. Nguyen (*) Faculty of Fundamental Sciences, Hue University of Medicine and Pharmacy, Hue University, Hue 530000, Vietnam e-mail: [email protected] T. A. T. Do Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam M. T. Man (*) Institute of Theoretical and Applied Research, Duy Tan University, Hanoi 100000, Vietnam e-mail: [email protected]
process. The detection and quantitation of the energy transfer mechanism might be to playing a critical role in improving spatial resolution, distance range, and spectral sensitivity for biophysical studies.
Keywords FRET . SET . Gold nanoparticle . Energy transfer . Donor-acceptor pair
Introduction To now, fluorescence resonance energy transfer (FRET) is being widely used in biophysical studies and other applications as the spectroscopic technique (Jennings and Strouse 2007; Medintz et al. 2005; Swierczewska et al. 2011; Anker et al. 2008; Lakowicz 2001; Dubertret et al. 2001; Chen et al. 2012). In typical FRET, the dipole-dipole coupling between the excited state of a donor fluorophore and another proximal ground-state acceptor, or two interacting biomolecules, that are individually labeled with donor and acceptor (Rasnik et al. 2005). The FRET’s distance dependence and association/or dissociation cause the conformational changes affecting the fluorescence from the donor (and acceptor) (Rasnik et al. 2005; Nolan and Lippard 2008; Wang et al. 2005). Currently, fluorescent dyes are widely used as donors in FRET. The combination of quantum dots and their unique optical properties as FRET participants (typically donors) have brought many of FRET’s highly successful biological applications (Ma et al. 2014). Gold nanoparticles as an acceptor in FRET
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have generated increasing research interests in exploiting their optical properties (Zhang et al. 2007). Besides, the FRET-based on the interaction betwee
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