Detection of Trypanosoma cruzi DNA in false negative samples of collected triatomines, xenodiagnosis material, and biops
- PDF / 396,151 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 6 Downloads / 253 Views
ORIGINAL ARTICLE
Detection of Trypanosoma cruzi DNA in false negative samples of collected triatomines, xenodiagnosis material, and biopsies of experimentally infected animals Leidi Herrera 1 & Cruz Manuel Aguilar 2,3 & Antonio Morocoima 4 & Mercedes Viettri 5 & María Lares 5 & Elizabeth Ferrer 5,6 Received: 23 August 2020 / Revised: 12 October 2020 / Accepted: 29 October 2020 # Springer Nature Switzerland AG 2020
Abstract Direct test over the gut material from triatomine vectors and xenodiagnosis over mammalian hosts are classical techniques for Trypanosoma cruzi parasitological diagnosis. Nevertheless, negative results can be a source of uncertainty. Experimental models have allowed evaluating the tissue invasion of different strains of T. cruzi, but conventional techniques for tissue biopsies involve time-consuming and elaborated procedures and have low sensitivity. Gut material of collected triatomines (microscopically negative) (n = 114), material of mammal xenodiagnoses (microscopically negative) (n = 138), and biopsy material (microscopically negative) from experimentally infected animals (n = 34) with isolates from endemic areas of Chagas’ disease from Venezuela were used for DNA extraction and PCR for the amplification of kinetoplast DNA (kDNA) and satellite DNA (sDNA) of T. cruzi. Positive PCR was observed in 53.6% of collected triatomine material, 15.8% of parasitological negative xenodiagnosis material, and 70.6% in biopsies, revealing underestimation by the parasitological tests and the valour of this analysis with preserved material. Anzoátegui was the state with the highest percentage of infection, and the triatomine species Rhodnius prolixus and Panstrongylus geniculatus had the highest percentages of infection. Didelphis marsupialis and Canis familiaris were the most infected by T. cruzi revealed by PCR of xenodiagnosis material. In addition, the PCR technique allowed demonstrating the invasion of T. cruzi in all tissues analyzed, constituting a molecular marker of tissue invasion. Keywords Trypanosoma cruzi . Triatomines . Xenodiagnosis . Biopsies . Experimental models . PCR
Introduction * Elizabeth Ferrer [email protected] 1
Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela
2
Centro de Investigaciones en Enfermedades Tropicales (CIET-UC), Facultad de Ciencias de la Salud, San Carlos, Cojedes, Venezuela
3
Universidad de Carabobo, Valencia, Venezuela
4
Centro de Medicina Tropical de Oriente, Universidad de Oriente (UDO) Núcleo Anzoátegui, Barcelona, estado Anzoátegui, Venezuela
5
Instituto de Investigaciones Biomédicas “Dr. Francisco J. Triana Alonso” (BIOMED), Universidad de Carabobo Sede Aragua, calle Cecilio Acosta, Urb. La Rinconada, Las Delicias, Maracay, estado Aragua, Venezuela
6
Departamento de Parasitología, Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, estado Aragua, Venezuela
American trypanosomiasis or Chagas’ disease is a metaxenic disease caused by
Data Loading...
