Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
- PDF / 162,564 Bytes
- 4 Pages / 595.276 x 790.866 pts Page_size
- 109 Downloads / 193 Views
MICROSATELLITE LETTERS
Development of 16 microsatellite loci for the endangered sandhill dunnart (Sminthopsis psammophila) Amanda L. McLean • Steven J. B. Cooper Melanie L. Lancaster • Susan M. Carthew
•
Received: 21 October 2013 / Accepted: 23 October 2013 / Published online: 4 December 2013 Ó Springer Science+Business Media Dordrecht 2013
Abstract Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach, and three loci, developed for Sminthopsis douglasi, were optimized for the endangered sandhill dunnart, Sminthopsis psammophila. Nineteen loci (16 S. psammophila and three S. douglasi) were tested on 38 individual S. psammophila from two populations in South Australia. Three loci deviated from Hardy–Weinberg equilibrium, possibly due to null alleles. No evidence of linkage disequilibrium was detected. We recommend a final panel of 17 loci for future genetic analyses of S. psammophila, which will aid in conservation management of this poorly understood and nationally endangered marsupial. Keywords Sminthopsis psammophila Endangered marsupial Microsatellites 454 sequencing
A. L. McLean (&) S. J. B. Cooper M. L. Lancaster S. M. Carthew School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA 5005, Australia e-mail: [email protected] A. L. McLean S. J. B. Cooper Australian Centre for Evolutionary Biology and Biodiversity, The University of Adelaide, Adelaide, SA 5005, Australia S. J. B. Cooper Evolutionary Biology Unit, South Australian Museum, North Terrace, Adelaide, SA 5000, Australia M. L. Lancaster Healesville Sanctuary, Badger Creek Road, Healesville VIC 3777, Australia S. M. Carthew Research Institute for Environment and Livelihoods, Charles Darwin University, Darwin, NT 0909, Australia
The sandhill dunnart Sminthopsis psammophila is a small insectivorous marsupial (30–55 g) found in arid and semiarid regions of Australia. Classified as nationally Endangered by the 2013 IUCN Red List, the species has experienced a 50 % range decline and is now known from three disjunct core populations; Eyre Peninsula, South Australia (33°12.9380 S, 137°3.0020 E); Yellabinna Regional Reserve, South Australia (30°49.9050 S, 132°25.8380 E); and Queen Victoria Springs Nature Reserve, Western Australia (29°58.8270 S, 124°00.8700 E) (unpublished Churchill 2001). S. psammophila is potentially threatened by introduced predators, changing fire regimes, habitat degradation and increased mining activities across its known distribution. Despite these threats, detailed management plans have not been implemented due to a lack of knowledge of the species. Our aim was to develop microsatellite loci for future studies of social and genetic structure in S. psammophila. A partial 454-shotgun sequencing approach on a Titanium GS-FLX platform at the Australian Genome Research Facility (Brisbane, Australia) was used to identify 624 microsatellite containing sequences from 11,406 sequenced reads as per Gardner et al. (2011). Twenty microsatellite loci and six Sm
Data Loading...
