Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-C
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LETTER
Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2 Renfei Lu1 • Xiuming Wu2 • Zhenzhou Wan3 • Yingxue Li2 • Lulu Zuo2 • Jianru Qin2,4 • Xia Jin5 Chiyu Zhang2
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Received: 19 February 2020 / Accepted: 22 March 2020 Ó Wuhan Institute of Virology, CAS 2020
Dear Editor, Since early December 2019, a large outbreak of pneumonia caused by a novel coronavirus (COVID-19) had emerged in Wuhan, China (Wu et al. 2020a, b; Zhou et al. 2020; Zhu et al. 2020; Jiang and Shi 2020). Similar to severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV), the new coronavirus also belongs to Betacoronavirus, and shares highest sequence identity to three SARS-like CoVs of bat origin (bat_CoV_RaTG13: 96.0%, bat-SLCoVZC45: 88.0% and bat-SL-CoVZXC21: 87.2%) (Zhou et al. 2020). Although only sharing about 79.5% genomic sequence identity to SARS-CoV, the new virus was demonstrate to use the same receptor angiotensin converting enzyme II (ACE2) for human infection as SARSCoV (Lu et al. 2020; Wu 2020; Zhou et al. 2020) and is officially named as SARS-CoV-2 (also known as 2019-nCoV) (Gorbalenya et al. 2020). Epidemically data showed that the virus has strong human-to-human Renfei Lu and Xiuming Wu have contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12250-020-00218-1) contains supplementary material, which is available to authorized users. & Chiyu Zhang [email protected] 1
Clinical Laboratory, Nantong Third Hospital Affiliated to Nantong University, Nantong 226006, China
2
Pathogen Discovery and Evolution Unit, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China
3
Medical Laboratory of Taizhou Fourth People’s Hospital, Taizhou 225300, China
4
College of Life Sciences, Henan Normal University, Xinxiang 453007, China
5
Shanghai Public Health Clinical Center, Fudan University, Jinshan District, Shanghai 201508, China
transmission ability, and it is spread by droplets produced by coughing and sneezing, infecting susceptible subjects through direct contacts and other possible transmission routes (e.g. fecal-mouth transmission) (Guan et al. 2020; Li et al. 2020). As of Mar. 27, 2020, the outbreak had resulted in 533,464 laboratory-confirmed cases, including 24,097 deaths around the world. Currently, there lacks suitable antiviral drugs or vaccines for SARS-CoV-2. Early, rapid, and reliable diagnostic assays for SARS-CoV-2 is of top priority to facilitate public health interventions that can reduce or avoid further spread of SARS-CoV-2 (Dennis Lo and Chiu 2020). Quantitative Real-time PCR (qPCR) is a robust technology for reliable laboratory diagnosis, and routinely used to detect causative pathogens. Since the release of genomic sequence of SARS-CoV-2 in Jan. 11, 2020, many TaqMan probe-based RT-qPCR assay
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