Development of dual-emission cluster of Ag atoms for genetically modified organisms detection
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ORIGINAL PAPER
Development of dual-emission cluster of Ag atoms for genetically modified organisms detection Mohsen Alipour 1,2 & Shirin Jalili 3 & Hadi Shirzad 3 & Ehsan Ansari Dezfouli 1 & Mohamad Hassan Fouani 1 & Amir Amiri Sadeghan 4 & Hassan Bardania 5,6 & Saman Hosseinkhani 1,7 Received: 6 January 2020 / Accepted: 7 October 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A DNA-silver nanocluster with two distinct emissions is devised, in which this unique modality has been exploited to develop a novel nanosensor for transgenic DNA detection. TEM and fluorescence analysis revealed the formation of Ag nanoclusters with a size of around 2 nm, which exhibit dual-emissions at 550 nm (green) and 630 nm (red). Moreover, in the presence of the target sequence (CaMV 35S promoter) from the transgenic plant, the nanoclusters showed an enhancement in the green emission and a reduction in the red emission. This property provided a ratiometric-sensing platform which lacks unavoidable noises. The ratio of green to red fluorescence emission (G/R) of the nanoclusters exhibited a linear relation with the target concentration in the range 10 to 1000 nM. However, the control DNA did not affect this ratio, which clearly confirmed the selective response of the designed nanosensor. This sensing platform had a detection limit of 1.5 nM and identified the DNA of transgenic soybeans within a short time. The mechanistic evaluation of the nanoclusters further revealed the role of protonated cytosine bases in the dual emission behavior. Finally, unique features of the designed nanosensor may improve the current approaches for the development and manufacturing of GMO detection tools. Keywords GMO detection . Ratiometric nanosensor . Silver nanocluster . Transgenic soybeans
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04591-2) contains supplementary material, which is available to authorized users. * Mohsen Alipour [email protected] * Saman Hosseinkhani [email protected] 1
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2
Department of Advanced Medical Sciences & Technologies, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
3
Research Institute of Police Science & Social Studies, Tehran, Iran
4
Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
6
Clinical Research Development Unit, Imamsajad Hospital, Yasuj University of Medical Sciences, Yasuj, Iran
7
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Genetically modified organisms (GMOs) are produced by introducing a transgene into the genome of an organism to achieve a desirable trait. The first GMO was flavor savor, long shelf-life tomato that has been produced by the insertion of antisense sequ
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