Differences in midgut transcriptomes between resistant and susceptible strains of Chilo suppressalis to Cry1C toxin
- PDF / 2,392,581 Bytes
- 19 Pages / 595.276 x 790.866 pts Page_size
- 72 Downloads / 268 Views
RESEARCH ARTICLE
Open Access
Differences in midgut transcriptomes between resistant and susceptible strains of Chilo suppressalis to Cry1C toxin Geng Chen1, Yanhui Wang1, Yanmin Liu1, Fajun Chen1* and Lanzhi Han2*
Abstract Background: Chilo suppressalis is a widespread rice pest that poses a major threat to food security in China. This pest can develop resistance to Cry toxins from Bacillus thuringiensis (Bt), threatening the sustainable use of insectresistant transgenic Bt rice. However, the molecular basis for the resistance mechanisms of C. suppressalis to Cry1C toxin remains unknown. This study aimed to identify genes associated with the mechanism of Cry1C resistance in C. suppressalis by comparing the midgut transcriptomic responses of resistant and susceptible C. suppressalis strains to Cry1C toxin and to provide information for insect resistance management. Results: A C. suppressalis midgut transcriptome of 139,206 unigenes was de novo assembled from 373 million Illumina HiSeq and Roche 454 clean reads. Comparative analysis identified 5328 significantly differentially expressed unigenes (DEGs) between C. suppressalis Cry1C-resistant and -susceptible strains. DEGs encoding Bt Cry toxin receptors, aminopeptidase-P like protein, the ABC subfamily and alkaline phosphatase were downregulated, suggesting an association with C. suppressalis Cry1C resistance. Additionally, Cry1C resistance in C. suppressalis may be related to changes in the transcription levels of enzymes involved in hydrolysis, digestive, catalytic and detoxification processes. Conclusion: Our study identified genes potentially involved in Cry1C resistance in C. suppressalis by comparative transcriptome analysis. The assembled and annotated transcriptome data provide valuable genomic resources for further study of the molecular mechanisms of C. suppressalis resistance to Cry toxins. Keywords: Chilo suppressalis, Transcriptome, Cry1C toxin, Difference, Resistance mechanism
Background The striped stem borer Chilo suppressalis Walker (Lepidoptera: Pyralidae) is a widespread rice pest that poses a major threat to food security throughout Asia, including China, India, Sri Lanka, Japan and Malaysia [1–3]. Excessive application of chemical pesticides has led to the rapid evolution of insect resistance [4]. Consequently, * Correspondence: [email protected]; [email protected] 1 Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China 2 The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
insect-resistant genetically modified rice expressing Bacillus thuringiensis (Bt) has been used as an alternative method to control rice stem borers. Indeed, insectresistant transgenic rice lines expressing Cry1A or Cry1C insecticidal proteins have been effective in controlling C. suppressalis [5–10]. In particular, the cry1C rice line has robust prospects for commercial use in China because it shows
Data Loading...
