Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples
- PDF / 311,697 Bytes
- 3 Pages / 612 x 792 pts (letter) Page_size
- 60 Downloads / 189 Views
HEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples M. Yu. Shkurnikova,*, S. A. Nersisyanb, c, A. Sh. Osepyanc, D. V. Maltsevab,d, and E. N. Knyazevd,** Presented by Academician S.I. Kolesnikov Received March 24, 2020; revised March 31, 2020; accepted March 31, 2020
Abstract—Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change intracellular regulatory mechanisms. In the framework of this study, it was shown that the quantitative estimates of inaccuracies in Drosha and Dicer activity significantly differ between the specimens of normal colon tissue and malignant colorectal tumors. Keywords: Drosha, Dicer, colorectal cancer, miRNA, microRNA, microRNA isoform, isomiR DOI: 10.1134/S1607672920040122
Cell models of the intestinal barrier in vitro make it possible to study various biological aspects, including the barrier, transport, and secretory functions of the intestinal epithelium, the interaction with the microbiome, and cell biology features under normal and pathological conditions. The use of immortalized tumor cell lines as models of human organs (e.g., human colorectal human adenocarcinoma Caco-2 cells as a model of the intestinal barrier [1]) allows obtaining well reproducible standardized results, excluding the contribution of many factors in a normal living organism that can barely be taken into account and reducing the variability inherent in the primary cell cultures [2]. The addition of extracellular matrix and the circulation of the nutrient medium in microfluidic devices additionally make the microenvironment of barrier models more similar to real conditions [3]. However, when studying the biological processes a Hertsen
Moscow Oncology Research Center, Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia b Faculty of Biology and Biotechnology, National Research University Higher School of Economics, Moscow, Russia c Faculty of Mechanics and Mathematics, Moscow State University, Moscow, Russia d Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia *e-mail: [email protected] **e-mail: [email protected]
in such models, the possible differences between the normal and tumor cells should always be borne in mind. Currently, the role of miRNAs in the regulation of the intestinal barrier is being actively studied. MicroRNAs are short RNA molecules approximately 22 nt long that play the key role in RNA interference [4]. Their most important function is the posttranscriptional suppression of the expression of target genes due to complementary binding of mi
Data Loading...
