Diosgenin Inhibits Excessive Proliferation and Inflammatory Response of Synovial Fibroblasts in Rheumatoid Arthritis by
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ORIGINAL ARTICLE
Diosgenin Inhibits Excessive Proliferation and Inflammatory Response of Synovial Fibroblasts in Rheumatoid Arthritis by Targeting PDE3B Roujun Wang,1 Yumeng Sun,2 Xiaowen Jin,2 Weibo Wen,3 and Yongjun Cao
2,4
(Received November 11, 2020; accepted November 20, 2020)
Abstract— Rheumatoid arthritis (RA) is a chronic inflammation that can lead to loss of range
of joint abnormalities in severe cases. Diosgenin has anti-inflammatory effects. This paper discussed the effect and mechanism of diosgenin on excessive proliferation and inflammatory response of synovial cells in RA. CCK-8 detected the cell viability, TUNEL assay detected the apoptosis of cells and western blot detected the expression of apoptosis-related proteins. Wound healing was used to detect cell migration and western blot detected the expression of migration-related proteins. ELISA kits were used to detect the levels of inflammatory cytokines in cells. Diosgenin can inhibit the proliferation and migration of RA synovial cells. At the same time, diosgenin could reduce the inflammatory response of RA synovial cells, during which the expression of PDE3B was significantly decreased. By overexpressing PDE3B, we found that diosgenin inhibited the proliferation, migration, and inflammatory response of RA synovial cells by downregulating PDE3B. Diosgenin can inhibit excessive proliferation and inflammatory response of synovial fibroblasts by targeting PDE3B. KEY WORDS: rheumatoid arthritis; diosgenin; cell proliferation; inflammatory response; synovial fibroblasts; PDE3B.
INTRODUCTION 1
Department of Diabetes and Endocrinology, Kunming Municipal Hospital of Traditional Chinese Medicine, Kunming, 650500, Yunnan, China 2 Department of Diabetes and Endocrinology, Affiliated Traditional Chinese Medicine Hospital of Nantong University, 41 Jianshe Road, Nantong City, 226001, Jiangsu, China 3 Department of Diabetes and Endocrinology, The No.1 Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming, 650021, Yunnan, China 4 To whom correspondence should be addressed at Department of Diabetes and Endocrinology, Affiliated Traditional Chinese Medicine Hospital of Nantong University, 41 Jianshe Road, Nantong City, 226001, Jiangsu, China. E-mail: [email protected]
Rheumatoid arthritis (RA) is an autoimmune disease characterized by persistent synovitis, systemic inflammation, and bone and cartilage erosion. Eventually, it can lead to stiffness and deformity of the joints [1]. The articular capsule in the human body’s articular cavity covers the articular cartilage surface, and the articular capsule can be divided into the inner layer and the outer layer. The outer fibrous layer is tough and thick and composed of dense connective tissue, while the inner synovial layer is thin and soft and composed of loose connective tissue. Synovial layer can secrete synovial fluid to lubricate
0360-3997/20/0000-0001/0 # 2020 Springer Science+Business Media, LLC, part of Springer Nature
Wang, Sun, Jin, Wen, and Cao nutrition and reduce the fri
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