Discriminating larvae of two syntopic Cychramus species (Coleoptera, Nitidulidae) by means of bar-HRM analysis

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Discriminating larvae of two syntopic Cychramus species (Coleoptera, Nitidulidae) by means of bar‑HRM analysis Lukas Zangl1,2,3   · Hannes Oberreiter1   · Herbert Huss4 · Edith Stabentheiner5   · Christian Sturmbauer1   · Stephan Koblmüller1  Received: 16 June 2020 / Accepted: 28 August 2020 © The Author(s) 2020

Abstract Molecular genetic methods are increasingly used to supplement or substitute classical morphology-based species identification. Here, we employ a COI mini-barcode coupled high-resolution melting analysis to quickly, cost-efficiently and reliably determine larvae of two closely related Cychramus (Coleoptera, Nitidulidae) species. Euclidean distance comparison (p