Distance-based quantification of miRNA-21 by the coffee-ring effect using paper devices
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ORIGINAL PAPER
Distance-based quantification of miRNA-21 by the coffee-ring effect using paper devices Dagan Zhang 1 & Chao Wu 2 & Chengxin Luan 3 & Peng Gao 1 & Huan Wang 1 & Junjie Chi 1 & Tiantian Kong 1 Received: 3 February 2020 / Accepted: 18 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Enabled by the coffee-ring effect, a paper-based signal transduce method is employed for catalytic hairpin assembly (CHA) amplification and hybridization chain reaction (HCR) to achieve miRNA quantification. Once the target miRNAs appeared, it was circularly used by CHA to initiate HCR amplification to produce a large number of G-quadruplex, which is combined with hemin to form a hemin/G-quadruplex DNAzyme. The DNAzyme catalyzes a colorimetric reaction to produce colored nanoparticles, which were converted to the end edge of the paper by evaporation-driven flow, forming a visible colored band. Higher concentration of miRNA led to more colored nanoparticles and thus a longer colored band that can simply be measured by a ruler. The results of determination of miRNA in samples demonstrate that the relative standard deviation of the proposed approach is 5.2%, highly sensitive and repeatable, with a working range 1.0 to 1000 pM and a LOD of 0.2 pM. The paper-based analytical device as a novel platform offers a new signal transduce pathway toward the detection of low-abundance biomarkers for diagnosis. Keywords Coffee-ring effect . Label-free . CHA and HCR amplification . miRNA
Introduction MicroRNA (miRNA) released from cells and organs are closely associated with the occurrence and development of cancers and have been recognized as a cancer biomarker, Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04500-7) contains supplementary material, which is available to authorized users. * Huan Wang [email protected] * Junjie Chi [email protected] * Tiantian Kong [email protected] 1
Guangdong Key Laboratory of Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China
2
Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biology and Food Engineering, Suzhou University, Suzhou 234000, China
3
Department of Hematology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui, China
which caused numbers of malignancy cancer diseases worldwide [1–6]. Therefore, the accurate and rapid detection of miRNA is key for early screening of cancer and monitoring the response of cancer therapy [7]. However, the quantitative detection of miRNA has remained a challenge because of low expression level in blood, especially in the early stage of cancer. To improve the sensitivity of cancer biomarker detection, intensive signal amplification methods have been developed including polymerase chain reaction (PCR), rolling circle amplification (RCA), loop-mediated isothermal mal amplification (LAMP), biobarcode, fluore
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