Distribution, DNA barcoding and genetic diversity of potato cyst nematodes in Indonesia
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Distribution, DNA barcoding and genetic diversity of potato cyst nematodes in Indonesia Nurul Dwi Handayani & Magali Esquibet & Josselin Montarry & Prabowo Lestari & Marjolein Couvreur & Antarjo Dikin & Johannes Helder & Eric Grenier & Wim Bert
Accepted: 14 July 2020 # Koninklijke Nederlandse Planteziektenkundige Vereniging 2020
Abstract Global trading of plant materials, in combination with agricultural practices, may facilitate the spreading of cyst nematodes to so far non-infected areas. Recently Potato Cyst Nematode (PCN) was recognized to be present in Indonesia and both diversity and distribution require further study. Assessment of PCN populations was done by collecting soil samples, determination of morphological characteristics in combination with ITS rDNA and COI mtDNA sequencing. Thirtyseven soil samples were collected from potato fields in the Indonesia archipelago. The results showed the presence of Globodera rostochiensis in 22 out of 37 sampling fields, namely North Sumatra (6 fields), Central
Java (12 fields), East Java (3 fields), and -for the first time- in Sulawesi (North Sulawesi) (1 field). The highest observed density was found in Banjarnegara (Central Java), i.e., 872 cysts 100 ml soil−1. Globodera pallida was not recovered. Both ITS and COI characterisation of Indonesian PCN (G. rostochiensis) revealed the virtual absence of sequence variation as compared to most PCN from the rest of the world; the COI sequences were identical to the most common and mostly distributed haplotype around the world. Microsatellite genotyping indicated a higher genetic diversity for populations from East Java than for populations from North Sumatra, suggesting that cysts at the origin of populations in
N. D. Handayani : P. Lestari : M. Couvreur : W. Bert (*) Nematology Research Unit, Department of Biology, Ghent University, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium e-mail: [email protected]
M. Esquibet e-mail: [email protected] J. Montarry e-mail: [email protected]
P. Lestari e-mail: [email protected] M. Couvreur e-mail: [email protected] N. D. Handayani (*) : P. Lestari Indonesian Agricultural Quarantine Agency, Ministry of Agriculture, E Building 5th Floor, Jl. Harsono RM. 3 Ragunan, Jakarta 12550, Indonesia e-mail: [email protected] M. Esquibet : J. Montarry : E. Grenier IGEPP, INRAE, Agrocampus-Ouest, Université de Rennes 1, 35650 Le Rheu, France
E. Grenier e-mail: [email protected] A. Dikin Directorate General of Estate Crops, Ministry of Agriculture, C Building, Jl. Harsono RM. 3 Ragunan, Jakarta 12550, Indonesia e-mail: [email protected] J. Helder Laboratory of Nematology, Wageningen University, Droevendaalsesteeg 1 RADIX building (107), 6708 PB, Wageningen, The Netherlands e-mail: [email protected]
Eur J Plant Pathol
North Sumatra were coming from populations in East Java. These data on species identification, population density, genetic diversity, and distribution of potato cyst nematode over the Indonesian archipelago constitute the v
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