DNA Metabarcoding to Assess the Diversity of Airborne Fungi Present over Keller Peninsula, King George Island, Antarctic
- PDF / 5,443,213 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 70 Downloads / 167 Views
FUNGAL MICROBIOLOGY
DNA Metabarcoding to Assess the Diversity of Airborne Fungi Present over Keller Peninsula, King George Island, Antarctica Luiz Henrique Rosa 1 & Otávio Henrique Bezerra Pinto 2 & Peter Convey 3 & Micheline Carvalho-Silva 4 & Carlos Augusto Rosa 1 & Paulo Eduardo Aguiar Saraiva Câmara 4 Received: 7 September 2020 / Accepted: 15 October 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract We assessed fungal diversity present in air samples obtained from King George Island, Antarctica, using DNA metabarcoding through high-throughput sequencing. We detected 186 fungal amplicon sequence variants (ASVs) dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota, and Chytridiomycota. Fungi sp. 1, Agaricomycetes sp. 1, Mortierella parvispora, Mortierella sp. 2, Penicillium sp., Pseudogymnoascus roseus, Microdochium lycopodinum, Mortierella gamsii, Arrhenia sp., Cladosporium sp., Mortierella fimbricystis, Moniliella pollinis, Omphalina sp., Mortierella antarctica, and Pseudogymnoascus appendiculatus were the most dominant ASVs. In addition, several ASVs could only be identified at higher taxonomic levels and may represent previously unknown fungi and/or new records for Antarctica. The fungi detected in the air displayed high indices of diversity, richness, and dominance. The airborne fungal diversity included saprophytic, mutualistic, and plant and animal opportunistic pathogenic taxa. The diversity of taxa detected reinforces the hypothesis that the Antarctic airspora includes fungal propagules of both intra- and inter-continental origin. If regional Antarctic environmental conditions ameliorate further in concert with climate warming, these fungi might be able to reactivate and colonize different Antarctic ecosystems, with as yet unknown consequences for ecosystem function in Antarctica. Further aeromycological studies are necessary to understand how and from where these fungi arrive and move within Antarctica and if environmental changes will encourage the development of non-native fungal species in Antarctica. Keywords Antarctic Peninsula . Ecology . Fungi . Metabarcoding . Taxonomy
Introduction The pristine environments of Antarctica offer unique opportunities to study how biological diversity disperses and
Supplementary Information The online version contains supplementary material available at https://doi.org/10.1007/s00248-02001627-1. * Luiz Henrique Rosa [email protected] 1
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
2
Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil
3
British Antarctic Survey, NERC, High Cross, Madingley Road, CB3 0ET Cambridge, UK
4
Departamento de Botânica, Universidade de Brasília, Brasília, Brazil
colonizes habitats under extreme conditions. Among the barriers that isolate Antarctica from other Southern Hemisphere landmasses (such as South America, Africa and Oceania) are the atmospheric circumpolar vortex (resulting in the consistent
Data Loading...
