Dual-emission of silicon nanoparticles encapsulated lanthanide-based metal-organic frameworks for ratiometric fluorescen
- PDF / 4,581,576 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 84 Downloads / 180 Views
ORIGINAL PAPER
Dual-emission of silicon nanoparticles encapsulated lanthanide-based metal-organic frameworks for ratiometric fluorescence detection of bacterial spores Dan Yang 1 & Shiliang Mei 1 & Zhuoqi Wen 2 & Xian Wei 1 & Zhongjie Cui 1 & Bobo Yang 2 & Chang Wei 1 & Yi Qiu 1 & Min Li 2 & Hui Li 3 & Wanlu Zhang 1 & Fengxian Xie 1 & Le Wang 2 & Ruiqian Guo 1,2 Received: 6 September 2020 / Accepted: 10 November 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Dipicolinic acid (DPA) is employed as a significant biomarker to detect Bacillus anthracis, which can do serious damages to the health of human beings. Hence, it is crucial to develop a fast and highly efficient strategy for DPA monitoring. In this work, based on silicon nanoparticles (Si NPs) and terbium metal-organic frameworks (Tb-MOFs), a hybrid structure (Si NPs/Tb-MOFs) as a novel dual-emitting fluorescence probe was fabricated for ratiometric detection of DPA, where blue light-emitting Si NPs (Ex: 280 nm; Em: 422 nm) are encapsulated into green light-emitting Tb-MOFs (Ex: 280 nm; Em: 547 nm). The optical properties and chemical composition of the as-obtained Si NPs/Tb-MOFs were characterized in detail. The Si NPs/Tb-MOFs probe not merely possesses the merits of a facile synthesis method but also is an excellent fluorescence probe. The response time towards DPA is less than 30 s, revealing that the process of detecting DPA can be completed in such a short time. The limit of detection for DPA is 5.3 nM, which is four orders of magnitude lower than an infectious dosage of anthrax spores for human beings (60 μM). This dual-emitting Si NPs/Tb-MOFs probe with interference-free and self-calibrating properties may be a potential candidate for further development in medical diagnosis. Keywords Metal-organic frameworks . Silicon nanoparticles . Dual-emitting . Fluorescence probe . Biomarker
Introduction Anthrax is a serious disease caused by Bacillus anthracis infection. Fast detection methods towards anthrax spores are crucial to minimize their damage to human beings. Dipicolinic acid (DPA), one of the main ingredients of anthrax
Dan Yang and Shiliang Mei contributed equally to this work. * Shiliang Mei [email protected] * Le Wang [email protected] * Ruiqian Guo [email protected] 1
Institute for Electric Light Sources, School of Information Science and Technology, Fudan University, Shanghai 200433, China
2
Institute of Future Lighting, Academy for Engineering and Technology, Fudan University, Shanghai 200433, China
3
Yanbian University Hospital, Yanji 133000, Jilin, China
spores, is considered as a significant biomarker in detection of anthracis diseases [1]. Various DPA detection methods have been reported in the past decades, including molecular imprinting [2], polymerase chain reaction (PCR) [3], surface plasmon resonance (SPR) [4], and surface enhanced Raman spectroscopy (SERS) [5]. Compared with other methods, fluorescence detection is more suitable for DPA detection due to its merits of excellent selectivity,
Data Loading...
