Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament
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BioMed Central
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Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells Masahiro Asano1,2, Satoshi Kubota1, Tohru Nakanishi1, Takashi Nishida1, Tomoichiro Yamaai3, Gen Yosimichi1, Kazumi Ohyama1, Tomosada Sugimoto3, Yoji Murayama2 and Masaharu Takigawa*1 Address: 1Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan, 2Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan and 3Department of Oral Functional Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Email: Masahiro Asano - [email protected]; Satoshi Kubota - [email protected]; Tohru Nakanishi - [email protected]; Takashi Nishida - [email protected]; Tomoichiro Yamaai - [email protected]; Gen Yosimichi - [email protected]; Kazumi Ohyama - [email protected]; Tomosada Sugimoto - [email protected]; Yoji Murayama - [email protected]; Masaharu Takigawa* - [email protected] * Corresponding author
Published: 05 October 2005 Cell Communication and Signaling 2005, 3:11
doi:10.1186/1478-811X-3-11
Received: 22 June 2005 Accepted: 05 October 2005
This article is available from: http://www.biosignaling.com/content/3/1/11 © 2005 Asano et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: CCN2/CTGF is known to be involved in tooth germ development and periodontal tissue remodeling, as well as in mesenchymal tissue development and regeneration. In this present study, we investigated the roles of CCN2/CTGF in the proliferation and differentiation of periodontal ligament cells (murine periodontal ligament-derived cell line: MPL) in vitro. Results: In cell cultures of MPL, the mRNA expression of the CCN2/CTGF gene was stronger in sparse cultures than in confluent ones and was significantly enhanced by TGF-β. The addition of recombinant CCN2/CTGF (rCCN2) to MPL cultures stimulated DNA synthesis and cell growth in a dose-dependent manner. Moreover, rCCN2 addition also enhanced the mRNA expression of alkaline phosphatase (ALPase), type I collagen, and periostin, the latter of which is considered to be a specific marker of the periosteum and periodontium; whereas it showed little effect on the mRNA expression of typical osteoblastic markers, e.g., osteopontin and osteocalcin. Finally, rCCN2/CTGF also stimulated ALPase activity and collagen synthesis. Conclusion: These results taken together suggest important roles of CCN2/CTGF in the development and regeneration of periodontal tissue incl
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