Effect of laparoscopic liver resection versus the open technique on hepatocyte regenerating activity in the rat
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and Other Interventional Techniques
Effect of laparoscopic liver resection versus the open technique on hepatocyte regenerating activity in the rat Petros Ypsilantis1 · Maria Lambropoulou2 · Konstantinos Anagnostopoulos3 · Eleni Panidou‑Tsoulou1 · Orestis Ioannidis1 · Albion Totsi1 · Michael Pitiakoudis1 · Constantinos Simopoulos1 Received: 2 July 2019 / Accepted: 11 November 2019 © Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Background Laparoscopic liver resection offers a safe and feasible option primarily for the excision of hepatic neoplasms. Timely recovery of liver volume is a key factor for improving prognosis and post-operative mortality of patients undergone liver resection. The aim of the present study was to compare liver regeneration after laparoscopic over open partial hepatectomy. Methods Wistar rats were subjected to laparoscopic 70% hepatectomy (group LAP-HEP), open 70% hepatectomy (group HEP), sham operation (group Sham) or no intervention (group Control). At various timepoints following operation (1 h–2 weeks), the liver was excised to assess relative liver weight, thiobarbituric acid reactive substances (TBARS) levels, mitotic activity, tissue expression of Nuclear Factor-κB (NFκB), Intercellular Adhesion Molecule-1 (ICAM-1) and Vascular Cell Adhesion Molecule-1 (VCAM-1) and the histopathologic profile. Results No differences were seen in relative liver weight between hepatectomy groups. Mitotic index was increased in all operative study groups, being higher in group LAP-HEP than in group HEP. TBARS levels were higher in group LAP-HEP compared to group HEP. NFκB and VCAM-1 tissue expression scores were increased in all operative study groups with VCAM-1 being higher in group HEP, while ICAM-1 was overexpressed only in hepatectomy groups. Mild histopathologic lesions were noted in hepatectomy groups with the histopathologic score being higher in group HEP (24 h). Conclusions Laparoscopic liver resection enhanced hepatocyte mitotic activity which was accompanied by mild oxidative stress and a less pronounced local inflammatory response and tissue injury to that of the open technique. Keywords Laparoscopy · Hepatectomy · Liver · Regeneration Laparoscopic liver resection (LLR) has been developed during the past two decades offering an alternative approach to liver surgeons for the excision of benign or malignant hepatic neoplasms and for hepatic parenchyma harvesting in living-donor liver transplantation. Clinical studies highlight the benefits of LLR over the open technique, which * Petros Ypsilantis [email protected] 1
Laboratory of Experimental Research and Surgical Research, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
2
Laboratory of Histology and Embryology, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
3
Laboratory of Biochemistry, School of Medicine, Democritus University of Thrace, Alexandroupolis, Greece
include less trauma and post-operative pain, shorter recovery period,
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