Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes
- PDF / 185,985 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 50 Downloads / 177 Views
ORIGINAL ARTICLE
Effect of long-term caffeine administration to mice on in vitro fertilization and embryo development using oocytes Hidemi Yokota • Yoshimasa Yokota • Mikako Yokota • Yasuyuki Araki • Yasuhisa Araki
Received: 10 January 2013 / Accepted: 29 April 2013 Ó Japan Society for Reproductive Medicine 2013
Abstract Purpose To evaluate the effect of long-term caffeine administration to mice on in vitro fertilization (IVF) of oocytes. Methods Mice were injected with different dosages (0, 0.1, and 1.0 mg/mouse/converted day) of caffeine for one month. Subsequently, the fertilization rate and embryo development to blastocyst stage were evaluated in IVF using oocytes from the mice. Results The retrieved average oocyte rate was significantly lower (27.4) in mice injected with 1.0 mg caffeine than in the control group (36.5; P \ 0.05); the fertilization rate was significantly different between the 0 mg (317/401; 79.1 %) and 1.0 mg group (199/301; 66.1 %) (P \ 0.05). At 96 h after insemination, the blastocyst formation rate was significantly decreased in the 1.0 mg group (94/199; 47.2 %) compared with the control (0 mg) group (237/317; 74.8 %) and 0.1 mg group (226/323; 70 %) (P \ 0.05). When 1.0 mg caffeine was administered for two weeks, embryo development was significantly impacted. Conclusions Our findings suggest that caffeine administration negatively impacts oocytogenesis and embryonic development after IVF. Keywords ART Blastocyst formation Caffeine Hatching In vitro fertilization
H. Yokota Y. Yokota (&) M. Yokota Yokota Maternity Hospital, 1-5-22 Shimokoide, Maebashi, Gunma 371-0031, Japan e-mail: [email protected] Y. Araki Y. Araki The Institute for Advanced Reproductive Medical Technology, 909-21 Ishii, Fujimi, Maebashi, Gunma 371-0105, Japan
Introduction In recent years, the pregnancy rate via assisted reproductive technology (ART) has been increasing for severely infertile women; however, even when normal patients without any specific abnormalities are treated with ART, many women do not achieve a successful pregnancy. One of the main infertility factors is advanced age. Furthermore, we are influenced by different environmental factors including food, pollution, and so on. There may be possible effects of common habitual caffeine ingestion on fertility among women. Caffeine is one of the most widely and routinely consumed pharmacologically active substances and is, in general, considered quite harmless to human beings [1, 2]. Furthermore, caffeine consumption is associated with benefits such as a decreased risk of endometrial cancer [3] and colorectal cancer [4]; however, women who habitually ingest caffeine have been found to have increased rates of spontaneous abortion, stillbirth, and low-birth-weight infants [5–9]. The literature contains only a handful of reports addressing the effect of caffeine intake on fecundity [10, 11]. In our previous pilot study (in preparation for submission), daily coffee intake among patients undergoing ART treatment was found to adversely impact
Data Loading...
