Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and
- PDF / 228,083 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 72 Downloads / 185 Views
REPRODUCTIVE MEDICINE
Effect of ovarian stimulation with recombinant follicle-stimulating hormone, gonadotropin-releasing hormone agonist and antagonists, on follicular fluid stem cell factor and serum urocortin 1 levels on the day of oocyte retrieval Onder Celik • Ebru Celik • Ercan Yilmaz • Nilu¨fer Celik • Ilgın Turkcuoglu • Mustafa Ulas Banu Kumbak • Erdal Aktan • I˙brahim Ozerol
•
Received: 8 July 2012 / Accepted: 12 March 2013 Ó Springer-Verlag Berlin Heidelberg 2013
Abstract Purpose To compare the serum and follicular fluid (FF) concentrations of stem cell factor (SCF) as well as the serum urocortin 1 (UCN1) concentration in gonadotropinreleasing hormone antagonist (GnRH-ant) and gonadotropin-releasing hormone agonist (GnRH-a) protocols for controlled ovarian hyperstimulation (COH) in IVF patients. Methods Follicular fluids and blood samples of 42 infertile women undergoing COH for IVF-embryo transfer with either GnRH agonist (n = 22) or GnRH antagonist (n = 20) protocols from 2010 to 2011 were collected during oocyte retrieval. SCF concentrations of serum and FF were assessed by sandwich enzyme immunoassay using ELISA Kit for SCF kid. Serum UCN1 concentration were measured using commercially available enzyme-linked immunosorbent assay. O. Celik (&) E. Celik E. Yilmaz I. Turkcuoglu Department of Obstetrics and Gynecology, Inonu University, Turgut Ozal Medical Center, 44069 Malatya, Turkey e-mail: [email protected] N. Celik Malatya State Hospital Biochemistry, Malatya, Turkey M. Ulas Department of Physiology, Firat University, Medical School, Elazig, Turkey B. Kumbak Department of Obstetrics and Gynecology, Firat University, Medical School, Elazig, Turkey E. Aktan Private Ege IVF center, Izmir, Turkey I˙. Ozerol Department of Microbiology, Inonu University, Medical Faculty, Malatya, Turkey
Results Concentrations of serum UCN1, serum and FF SCF were similar in the two groups. The serum SCF levels correlated strongly with the follicular SCF levels (r = 0.770, p \ 0.001). The mean implantation rate, biochemical and clinical pregnancy rate and live birth rate per cycle were also similar in the groups. Conclusions These observations suggest that there is no significant difference in follicular microenvironment in terms of SCF and UCN1 between agonist and antagonist protocols. Keywords Assisted reproduction Oocyte maturation SCF UCN1 GnRH agonist GnRH antagonist
Introduction Gonadotropin-releasing hormone (GnRH) agonist and antagonist protocols have been widely used treatments for ovarian stimulation in IVF patients. Yet, contributing factors such as ovulation induction agents to the onset of primordial follicle development and endometrial maturation are not well understood. Although a slightly lower pregnancy rate, comparing agonist and antagonist cycles, is controversial, most studies suggested decreased serum estradiol levels on the day of hCG administration and diminished number of mature oocytes during retrieval in the GnRH-ant cycle in comparison to GnRH-a cycle [1, 2]. Stem cell
Data Loading...
