Effect of Periodontal Pathogens on Total Bone Volume Fraction: A Phenotypic Study
- PDF / 3,741,955 Bytes
- 8 Pages / 595.276 x 841.89 pts (A4) Page_size
- 37 Downloads / 113 Views
40(4):753-760,2020
753
Effect of Periodontal Pathogens on Total Bone Volume Fraction: A Phenotypic Study* Zai-yu ZHANG1, 2†, Meng-ru XIE1†, Yi LIU1, Yao-xu LI1, Ke WU3, Yu-mei DING1# 1 Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2 Department of Stomatology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China 3 Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Huazhong University of Science and Technology 2020
Summary: Studies have shown that periodontal pathogens can enter the bloodstream, causing a series of reactions that can lead to a variety of systemic diseases. Epidemiological investigations also found a tight correlation between periodontitis (PD) and osteoporosis. This study aimed to further explore the effect of periodontal pathogens on bone volume fraction like bone tissue and mass, and explain the relationship between PD and osteoporosis. Sprague Dawley rats (female, 16 weeks old) were divided into the wild-type (WT) control group (n=9) and PD group (n=9). After eight weeks, periodontal tissues and ligatures, the fourth lumbar vertebra, the femur, the tibia, and blood were extracted and analyzed by micro-computed tomography (micro-CT), hematoxylin and eosin (H&E) staining, tartrate-resistant acid phosphatase (TRAP) staining, polymerase chain reaction (PCR), and enzyme-linked immunoassay (ELISA), respectively. We found that the bone mass of the lumbar vertebra, femur, and tibia was decreased in the PD group. The number of osteoclasts was higher in bone tissue in the PD group than in the WT group (P
Data Loading...
