Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats
- PDF / 1,780,412 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 81 Downloads / 181 Views
(2019) 14:259
RESEARCH ARTICLE
Open Access
Effects of ginsenoside Rb1 on spinal cord ischemia-reperfusion injury in rats Jin-tao Ye1, Feng-tao Li1, Sheng-li Huang1, Jian-li Xue1, Yirixiati Aihaiti2, Hao Wu1, Ruo-xi Liu1 and Bin Cheng1*
Abstract Background: The aim of this study was to evaluate the effects of different doses of ginsenoside Rb1 (GRb1) pretreatment on spinal cord ischemia-reperfusion (SCII) in rats and explore the potential mechanisms about the expression of survivin protein after the intervention. Methods: A total of 90 healthy adult Sprague-Dawley (SD) rats were randomly divided into six groups: sham-operated (n = 15), SCII model (n = 15), and GRb1-treated groups (n = 60). The GRb1-treated group was divided into four subgroups: 10 mg/kg, 20 mg/kg, 40 mg/kg, and 80 mg/kg (n = 15). The corresponding dose of GRb1 was injected intraperitoneally 30 min before operation and every day after operation. Forty-eight hours after model establishment, the neurological function of hind limbs was measured with Basso, Beattie, and Bresnahan (BBB) scale. The superoxide dismutase (SOD) and malondialdehyde (MDA) levels in serum and spinal cord tissue were detected respectively. The expression of survivin protein was observed by immunofluorescence staining. HE and TUNEL staining were used to observe neural cell injury and apoptosis, respectively, in the spinal cord of rats with SCII. Results: The intervention of different doses of GRb1 could increase SOD activity and decrease MDA content in serum and spinal cord tissue, increase survivin protein expression, and decrease neuronal apoptosis. It was dose-dependent, but there was no significant change between 40 mg/kg and 80 mg/kg. Conclusions: GRb1 could reduce the cell apoptosis induced by SCII through inhibiting oxidative stress. It can also inhibit apoptosis by promoting the expression of Survivin protein. Ginsenoside Rb1 had a dose-dependent protective effect on SCII in the dose range of 10 mg/kg–40 mg/kg. Keywords: Spinal cord Injury, Ischemia-reperfusion Injury, Oxidative stress, Ginsenoside Rb1, Survivin protein
Introduction Spinal cord ischemia-reperfusion injury (SCII) refers to the phenomenon that the nerve function could not be recovered after a period of ischemia and blood reperfusion, on the contrary, the nerve function was damaged and aggravated [1, 2]. SCII could occur in a variety of conditions, such as spinal trauma, vascular surgery, and the like. Spinal cord tissue belonged to nerve tissue. So far, most studies still believe that nerve tissue ischemiareperfusion injury was a disease without effective treatment. At present, the general treatment method was that once SCII is found, immediate treatment with large doses of methylprednisolone and neurotrophic drugs * Correspondence: [email protected] 1 Department of Orthopaedics, Xi’an Jiaotong University Second Affiliated Hospital, No. 157 Xiwu Road, Xi’an 710004, Shaanxi Province, People’s Republic of China Full list of author information is available at the end of the article
could promote the re
Data Loading...
