Effects of low temperature on photoinhibition and singlet oxygen production in four natural accessions of Arabidopsis
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ORIGINAL ARTICLE
Effects of low temperature on photoinhibition and singlet oxygen production in four natural accessions of Arabidopsis Heta Mattila1 · Kumud B. Mishra2 · Iiris Kuusisto1 · Anamika Mishra2 · Kateřina Novotná2 · David Šebela2 · Esa Tyystjärvi1 Received: 20 December 2019 / Accepted: 8 July 2020 © The Author(s) 2020
Abstract Main conclusions Low temperature decreases PSII damage in vivo, confirming earlier in vitro results. Susceptibility to photoinhibition differs among Arabidopsis accessions and moderately decreases after 2-week cold-treatment. Flavonols may alleviate photoinhibition. Abstract The rate of light-induced inactivation of photosystem II (PSII) at 22 and 4 °C was measured from natural accessions of Arabidopsis thaliana (Rschew, Tenela, Columbia-0, Coimbra) grown under optimal conditions (21 °C), and at 4 °C from plants shifted to 4 °C for 2 weeks. Measurements were done in the absence and presence of lincomycin (to block repair). PSII activity was assayed with the chlorophyll a fluorescence parameter Fv/Fm and with light-saturated rate of oxygen evolution using a quinone acceptor. When grown at 21 °C, Rschew was the most tolerant to photoinhibition and Coimbra the least. Damage to PSII, judged from fitting the decrease in oxygen evolution or Fv/Fm to a first-order equation, proceeded more slowly or equally at 4 than at 22 °C. The 2-week cold-treatment decreased photoinhibition at 4 °C consistently in Columbia-0 and Coimbra, whereas in Rschew and Tenela the results depended on the method used to assay photoinhibition. The rate of singlet oxygen production by isolated thylakoid membranes, measured with histidine, stayed the same or slightly decreased with decreasing temperature. On the other hand, measurements of singlet oxygen from leaves with Singlet Oxygen Sensor Green suggest that in vivo more singlet oxygen is produced at 4 °C. Under high light, the PSII electron acceptor Q A was more reduced at 4 than at 22 °C. Singlet oxygen production, in vitro or in vivo, did not decrease due to the cold-treatment. Epidermal flavonols increased during the cold-treatment and, in Columbia-0 and Coimbra, the amount correlated with photoinhibition tolerance. Keywords Acclimation · Charge recombination · Chilling stress · Cold-hardening · Photodamage · Photoinactivation · Reactive oxygen species · SOSG
Communicated by Dorothea Bartels. Heta Mattila and Kumud B. Mishra contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03423-0) contains supplementary material, which is available to authorized users. * Esa Tyystjärvi [email protected] 1
Department of Biochemistry, Molecular Plant Biology, University of Turku, 20014 Turku, Finland
Global Change Research Institute of the Czech Academy of Sciences, Bělidla 986, 4a, Brno 603 00, Czech Republic
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Abbreviations CD Developed at cold (grown 15 weeks at 4 °C) CT Cold-treated (grown 6 weeks at 21 °C and 2 weeks at 4 °C) DCMU 3-(3,4-Dichlorophenyl)-1,1-dimethyl
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