Effects of radiofrequency field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the b
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ORIGINAL PAPER
Effects of radiofrequency field exposure on proteotoxic-induced and heat-induced HSF1 response in live cells using the bioluminescence resonance energy transfer technique Emmanuelle Poque 1 & Hermanus J. Ruigrok 2 & Delia Arnaud-Cormos 3,4 & Denis Habauzit 5 & Yann Chappe 2 & Catherine Martin 5 & Florence Poulletier De Gannes 2 & Annabelle Hurtier 2 & André Garenne 2 & Isabelle Lagroye 2,6 & Yves Le Dréan 5 & Philippe Lévêque 3 & Yann Percherancier 2 Received: 27 March 2020 / Revised: 9 October 2020 / Accepted: 12 October 2020 # Cell Stress Society International 2020
Abstract As of today, only acute effects of RF fields have been confirmed to represent a potential health hazard and they are attributed to non-specific heating (≥ 1 °C) under high-level exposure. Yet, the possibility that environmental RF impact living matter in the absence of temperature elevation needs further investigation. Since HSF1 is both a thermosensor and the master regulator of heatshock stress response in eukaryotes, it remains to assess HSF1 activation in live cells under exposure to low-level RF signals. We thus measured basal, temperature-induced, and chemically induced HSF1 trimerization, a mandatory step on the cascade of HSF1 activation, under RF exposure to continuous wave (CW), Global System for Mobile (GSM), and Wi-Fi-modulated 1800 MHz signals, using a bioluminescence resonance energy transfer technique (BRET) probe. Our results show that, as expected, HSF1 is heat-activated by acute exposure of transiently transfected HEK293T cells to a CW RF field at a specific absorption rate of 24 W/ kg for 30 min. However, we found no evidence of HSF1 activation under the same RF exposure condition when the cell culture medium temperature was fixed. We also found no experimental evidence that, at a fixed temperature, chronic RF exposure for 24 h at a SAR of 1.5 and 6 W/kg altered the potency or the maximal capability of the proteasome inhibitor MG132 to activate HSF1, whatever signal used. We only found that RF exposure to CW signals (1.5 and 6 W/kg) and GSM signals (1.5 W/kg) for 24 h marginally decreased basal HSF1 activity. Keywords HSF1 . Bioluminescence resonance energy transfer . Radiofrequency . Trimerization
Emmanuelle Poque and Hermanus J. Ruigrok contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12192-020-01172-3) contains supplementary material, which is available to authorized users. * Yann Percherancier [email protected] 1
CNRS, Bordeaux INP, CBMN laboratory, UMR5248, Bordeaux University, F-33607 Pessac, France
2
CNRS, IMS laboratory, UMR5218, Bordeaux University, F-33400 Talence, France
3
CNRS, XLIM, UMR 7252, Limoges University, F-87000 Limoges, France
4
Institut Universitaire de France (IUF), F-75005 Paris, France
5
Institut de Recherche en Santé, Environnement et Travail (IRSET) – UMR_S 1085, Rennes University, F-35000 Rennes, France
6
Paris Sciences et Lettres Research University, F-75006 Paris, France
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