Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase I
- PDF / 2,153,912 Bytes
- 11 Pages / 595.276 x 785.197 pts Page_size
- 11 Downloads / 191 Views
RESEARCH ARTICLE
Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes Ying Huo1,2,3,4,*, Peng Yuan1,3,4,*, Qingyuan Qin1,3,4,*, Zhiqiang Yan1,3,4,5, Liying Yan1,3,4,6, Ping Liu1,3,4, Rong Li1,3,4,6,
Jie Yan (✉)1,3,4, Jie Qiao1,3,4,5,6 1
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China; Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing 100191, China; 3Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, China; 4Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China; 5Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China; 6 National Clinical Research Center of Obstetrics and Gynecology, Beijing 100191, China 2
© Higher Education Press 2020
Abstract Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies have demonstrated that conventional slow-freezing cryopreservation procedures, but not storage time, can alter the gene expression profiles of frozen oocytes. Whether vitrification procedures and the related frozen storage durations have any effects on the transcriptomes of human metaphase II oocytes remain unknown. Four women (30–32 years old) who had undergone IVF treatment were recruited for this study. RNA-Seq profiles of 3 fresh oocytes and 13 surviving vitrified-thawed oocytes (3, 3, 4, and 3 oocytes were cryostored for 1, 2, 3, and 12 months) were analyzed at a single-cell resolution. A total of 1987 genes were differentially expressed in the 13 vitrifiedthawed oocytes. However, no differentially expressed genes were found between any two groups among the 1-, 2-, 3-, and 12-month storage groups. Further analysis revealed that the aberrant genes in the vitrified oocytes were closely related to oogenesis and development. Our findings indicated that the effects of vitrification on the transcriptomes of mature human oocytes are induced by the procedure itself, suggesting that long-term cryostorage of human oocytes is safe. Keywords
human metaphase II oocyte; vitrification; cryostorage duration; single-cell RNA-Seq; lncRNA
Introduction Oocyte cryopreservation has become a clinically viable option for women who are facing age-related or iatrogenic decreases in both the quality and quantity of their oocytes because of declining oocyte quality, invasive cancer treatment, premature ovarian failure, and polycystic ovary syndrome [1]. Oocyte cryopreservation is a breakthrough technique for women who elect to delay childbearing, require gonadotoxic therapy, or perform egg donation [2–4].
Received January 2, 2020; accepted April 17, 2020 Correspondence: Jie Yan, [email protected] *
These authors contributed equally to this work.
The first human oocyte reported to be cryopreserved was preserved via the slow-freezing technique, in which the rate of cooling can be programmed in l
Data Loading...
