EGFR Gene Mutation and Methodological Evaluation in 399 Patients with Non-small Cell Lung Cancer
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EGFR Gene Mutation and Methodological Evaluation in 399 Patients with Non-small Cell Lung Cancer* Hong-yun ZHENG1, Hai-bo WANG1, Fu-jin SHEN2, Yong-qing TONG1, Qian YAO1, Bin QIAO1, Si SUN1, Yan LI1# 1 Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan 430060, China 2 Department of Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China Huazhong University of Science and Technology 2020
Summary: The purpose of the present study was to study the characteristics of epidemic growth factor receptor (EGFR) gene distribution in patients with non-small cell lung cancer (NSCLC), and to detect the mutation rate of EGFR gene by Sanger sequencing and amplification refractory mutation system (ARMS)-PCR. Paraffin-embedded sections of NSCLC tissues from 399 NSCLC patients diagnosed in Renmin Hospital of Wuhan University were collected, 103 of them were detected for exons 18–21 mutation of EGFR by Sanger sequencing method, 296 cases were detected for exons 18–21 mutation by ARMS-PCR method. DNA extraction of both groups was performed with Qiagen QLAamp DNA FFPE Tissue KIT. Comparisons of detection rates between the two methods were conducted by row X list chi-square test. The total mutation rate of EGFR gene detected by Sanger sequencing was 21.4%, exons 18–21 and combined mutation rates were 1.0%, 9.7%, 1.0%, 7.8% and 2.0%, respectively. And the proportions were 4.7%, 45.2%, 4.7%, 36.3% and 9.4% respectively. The total mutation rate detected by ARMS-PCR was 51.4%, exons 18–21 and combined mutation rates were 2.7%, 27%, 1.7%, 18.2% and 1.7%, respectively. The proportions were 5.3%, 52.6%, 3.3%, 35.5% and 3.3% respectively. Further analysis of mutation rate showed that there was significant difference between the two methods in detecting total mutation of EGFR gene (P
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