Enzymatic Modulators from Induratia spp.
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Enzymatic Modulators from Induratia spp. Andréa Patrícia da Silva Pomposo Bastos1 · Patrícia Gomes Cardoso2 · Ítalo Augusto Férrer Melo Santos2 · Marcus Vinicius Cardoso Trento3 · Laura Cristina Jardim Porto1 · Silvana Marcussi3 Received: 6 March 2020 / Accepted: 19 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In the present work, ethyl acetate extracts, consisting of non-volatile compounds, from the culture of endophytic fungi isolated from coffee plants, Induratia coffeana and Induratia yucatanensis, were prospected in enzyme modulation tests that act in human hemostasis. Dry extracts of the fungi were diluted in dimethyl sulfoxide p.a. 99.9% (DMSO), and then tested. Bothrops atrox venom was used as an enzyme source and tool to induce the activities. Prior to the evaluation of the activities, incubations of the extracts with the venom were performed in the proportions 1: 0.01, 1: 0.25, 1: 0.5, and 1: 1 (venom: extract; mass: mass). The extracts of all fungi promoted a significant increase in the clotting time induced by the venom, which was even longer when the extracts were previously incubated with the citrated plasma. The activity of phospholipases A2 did not significantly change when evaluated in the presence of fungal extracts. However, the evaluated extracts inhibited proteases by 73% and 30% in the thrombolytic and caseinolytic tests, respectively. In addition, the extracts did not induce cytotoxicity on human erythrocytes when evaluated in the absence of the venom. Thus, it is possible to suggest the presence of specific interactions between molecules present in extracts of Induratia spp. and venom proteases, highlighting non-volatile metabolites as promising sources of compounds of medical and scientific interest.
Introduction
* Patrícia Gomes Cardoso [email protected] Andréa Patrícia da Silva Pomposo Bastos [email protected] Ítalo Augusto Férrer Melo Santos [email protected] Marcus Vinicius Cardoso Trento [email protected] Laura Cristina Jardim Porto [email protected] Silvana Marcussi [email protected] 1
Nutrition Department (Departamento de Nutrição), Universidade Federal de Lavras (UFLA), Lavras, MG 37200‑900, Brazil
2
Biology Department (Departamento de Biologia), BIOGEN Laboratory (Laboratório BIOGEN), Universidade Federal de Lavras (UFLA), Lavras, MG 37200‑900, Brazil
3
Chemistry Department (Departamento de Química), Biochemistry Laboratory (Laboratório de Bioquímica), Universidade Federal de Lavras (UFLA), Lavras, MG 37200‑900, Brazil
Microorganisms are a potential source of new bioactive metabolites that can be used in human health. The endophytic fungi isolated from different plants, mainly medicinal plants with the capacity to produce a range of biologically active metabolites, shifted the focus of new drug sources from plants to fungi [1]. One advantage in producing functional fungal metabolites is the possibility of large-scale and short time cultivation, usually using substrates of low cost. Steroids, alkal
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