Enzyme-Mediated Ligation Methods
This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover t
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Timo Nuijens Marcel Schmidt Editors
Enzyme-Mediated Ligation Methods
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Enzyme-Mediated Ligation Methods Edited by
Timo Nuijens and Marcel Schmidt EnzyPep B.V., Geleen, The Netherlands
Editors Timo Nuijens EnzyPep B.V. Geleen, The Netherlands
Marcel Schmidt EnzyPep B.V. Geleen, The Netherlands
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9545-5 ISBN 978-1-4939-9546-2 (eBook) https://doi.org/10.1007/978-1-4939-9546-2 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The increasing understanding of biological systems provides exciting novel opportunities for their modulation using a wealth of therapeutic modalities. Whereas drug discovery has traditionally focused on small-molecule drugs, the advances in life science over the past 30 years have led to a significant expansion of this focus toward larger molecules beyond Lipinski’s rule of five, such as peptide or protein-based therapeutics. The emergence of this class of larger biological macromolecules as successful therapeutics has been accompanied by a complementary need of methodologies to enable their synthesis, derivatives thereof (e.g., containing unnatural moieties), or hybrid-modality conjugates (e.g., antibody-drug conjugates). Whereas initial efforts to modify peptides and proteins mostly focused on traditional chemical ligation methodologies such as native che
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