Establishment of animal model manifested as bladder neurogenic changes generated by bilateral pelvic nerve injury in mal
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UROLOGY - ORIGINAL PAPER
Establishment of animal model manifested as bladder neurogenic changes generated by bilateral pelvic nerve injury in male rats Qingyu Ge1 · Meiduo Wang2 · Yao Lin3 · Congyun Xu3 · Jun Xiao1 · Zhou Shen3 Received: 21 July 2020 / Accepted: 25 September 2020 © Springer Nature B.V. 2020
Abstract Purpose To establish a male rat model of neurogenic bladder after bilateral pelvic nerve injury (BPNI) and investigate the factors associated with onset of neurogenic bladder. Methods Twenty-four 8-week-old male Sprague–Dawley rats were randomly divided into three groups (n = 8 rats per group). Rats in 4-week and 8-week nerve injury group underwent BPNI, while rats in the sham group underwent a sham operation. Bladder functional analysis were performed and then bladders tissues were harvested for morphological examination and investigating the mRNA expression levels of target genes in all rats. Results The bladder weight significantly increased in rats following BPNI. Functional analysis revealed non-voiding contractions and decreased detrusor contractility following BPNI, manifested as elevated post-void residual and bladder capacity while reduced maximum voiding pressure and voiding efficiency. The collagen area in bladder tissue and mRNA expression levels of target genes significantly increased at 4 or 8 weeks post-BPNI except Smad3. At 4 weeks post-BPNI, expression levels of vesicular acetylcholine transporter were reduced, then returned to baseline at 8 weeks. Expression levels of tyrosine hydroxylase were reduced at both 4 and 8 weeks post-BPNI. Conclusions A neurogenic bladder animal model was successfully established by performing BPNI in male rats, characterized by impaired voiding function, bladder detrusor fibrosis, and reduced neurotransmitter release. Keywords Urinary bladder · Neurogenic · Pelvis · Urination disorders · Pelvic radical surgery
Introduction Neurogenic bladder is a very broad disease definition that encompasses various disease and injury states affecting the bladder, which usually performed on the premise of * Jun Xiao [email protected] * Zhou Shen [email protected] 1
Department of Urology, Provincial Hospital Affiliated to Anhui Medical University, Anhui Medical University, Hefei 230001, Anhui, People’s Republic of China
2
Clinical Skills Training Center, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei 230001, Anhui, People’s Republic of China
3
Department of Urology, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei 230001, Anhui, People’s Republic of China
neuropathy before diagnosis. Depending on the degree and location of the lesion, the neurogenic bladder exhibits multitudinous clinical manifestations. In recent years, with the progress of technology, an increasing number of patients with pelvic tumors (e.g., ovarian cancer, rectal cancer, and prostate cancer) can be identified a
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