Estrogen Regulates Duodenal Calcium Absorption Through Differential Role of Estrogen Receptor on Calcium Transport Prote
- PDF / 2,080,981 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 174 Views
ORIGINAL ARTICLE
Estrogen Regulates Duodenal Calcium Absorption Through Differential Role of Estrogen Receptor on Calcium Transport Proteins Xubiao Nie1,2 · Hai Jin1,2,3 · Guorong Wen1,2,3 · Jingyu Xu1,2,3,4 · Jiaxing An1,2,3 · Xuemei Liu1,2,3 · Rui Xie1,2,3 · Biguang Tuo1,2,3 Received: 3 September 2019 / Accepted: 12 January 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background and Aims Intestinal calcium absorption from the diet plays important role in maintaining calcium homeostasis in the body. Estrogen exerts wide physiological and pathological effects in the human. Previous studies have shown that estrogen is involved in the intestinal calcium absorption. In this study, we made investigation on the mechanism of estrogen action on duodenal calcium absorption. Methods The experiments were performed in mice, human, and human duodenal epithelial cells, SCBN cells. Murine duodenal calcium absorption was measured by using single pass perfusion of the duodenum in vivo. The calcium absorption of SCBN cells was evaluated by calcium imaging system. The expression of calcium transport proteins, transient receptor potential cation channel (TRPV6) and plasma membrane calcium pump (PMCA1b), in the duodenum or SCBN cells were analyzed by western blot. Results The duodenal calcium absorption in ovariectomized mice was significantly decreased, compared with control female mice, which returned to control level after 17β-estradiol replacement treatment. Estrogen regulated the expressions of TRPV6 and PMCA1b in murine and human duodenal mucosae and SCBN cells. The further results from SCBN cells showed that 17β-estradiol regulated calcium influx through the respective effects of estrogen receptor (ER) ɑ and β on TRPV6 and PMCA1b. Conclusion Estrogen regulates duodenal calcium absorption through differential role of ERɑ and ERβ on duodenal epithelial cellular TRPV6 and PMCA1b. The study further elucidates the mechanism of estrogen on the regulation of intestinal calcium absorption. Keywords Calcium absorption · Calcium transport proteins · Duodenum · Estrogen · Estrogen receptor
Introduction
Xubiao Nie and Hai Jin have contributed equally to this work. * Biguang Tuo [email protected] 1
Department of Gastroenterology, Affiliated Hospital, Zunyi Medical University, 149 Dalian Road, Zunyi 563003, China
2
Digestive Disease Institute of Guizhou Province, Zunyi, China
3
Clinical Medical Research Center of Digestive Diseases of Guizhou Province, Zunyi, China
4
Department of Gastroenterology and Hepatology, Hannover Medical School, Hannover, Germany
Calcium, the most abundant cation in the human body, is involved in organizing the bone and teeth and is a critical cation in both the extracellular and intracellular spaces [1]. Calcium balance is tightly regulated by the concerted action of calcium absorption in the intestine, reabsorption in the kidney, and exchange from the bone, to maintain serum calcium within a narrow range. Dietary intake is the only source by which
Data Loading...
