Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

            This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters foc

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Narendra Wajapeyee Romi Gupta Editors

Eukaryotic Transcriptional and Post-transcriptional Gene expression Regulation

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation Edited by

Narendra Wajapeyee and Romi Gupta Department of Pathology, Yale University School of Medicine, New Haven, CT, USA

Editors Narendra Wajapeyee Department of Pathology Yale University School of Medicine New Haven, CT, USA

Romi Gupta Department of Pathology Yale University School of Medicine New Haven, CT, USA

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6516-8 ISBN 978-1-4939-6518-2 (eBook) DOI 10.1007/978-1-4939-6518-2 Library of Congress Control Number: 2016958307 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A

Preface Regulation of gene expression at the transcriptional and posttranscriptional level is essential for all eukaryotic cells for maintaining their survival and cell identity. Eukaryotic cells have developed intricate and complex control mechanisms that allow them to determine which genes to express and to what extent in a given cell type. Therefore, a comprehensive study of the transcriptional and posttranscriptional mechanisms of gene regulation is expected to provide key insights into almost all of the important biological processes. This new edition of Methods in Molecular Biology on Eukaryotic Transcriptional and Posttranscriptional Gene Expression Regulation compiles a variety of very useful protocols that will allow