Evaluation of the Effect of Plasma from Patients with Trophic Ulcers on the Function of Dermal Fibroblasts, Mesenchymal
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Cell Technologies in Biology and Medicine, No. 2, August, 2020
Evaluation of the Effect of Plasma from Patients with Trophic Ulcers on the Function of Dermal Fibroblasts, Mesenchymal Stem Cells, and Endothelial Cells A. P. Lykov1,2, M. A. Surovtseva1,2, O. V. Poveshchenko1,2, N. A. Bondarenko1,2, I. I. Kim1,2, and E. V. Yankaite1
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 98-19, June, 2020 Original article submitted April 23, 2019 We studied the effect of platelet lysate and platelet-poor plasma from patients with trophic ulcers with and without type 2 diabetes mellitus on proliferation, migration, and apoptosis of human dermal fibroblast, mesenchymal stem cells, and endothelial cells. It is shown that plasma obtained from patients with type 2 diabetes mellitus produced inhibitory effects. Key Words: plasma; fibroblast; mesenchymal stem cells; endothelial cell line EA.hy 926 High incidence of trophic ulcers (1-4%), tendency to relapse and expansion of the lesion area, low efficiency of traditional methods of treatment, and high cost of treatment necessitate the search for new methods of therapy of this pathology [6,7]. A promising trend in the treatment of trophic ulcers is the use of cell technologies. We have shown the effectiveness of the use of biomedical cell product in acute myocardial infarction, chronic inflammation in the intestine, and burn skin wound in animals, as well as in age-related macular degeneration in humans [8,9,12,13,15,23]. Dermal fibroblasts have found their application for dermoplasty of trophic ulcers [21]. Encouraging results were also observed after application of plateletrich autologous plasma due to high content of growth factors [1-5,16]. However, the effect of blood plasma in patients with trophic ulcers with and without diabetes mellitus on the functional properties of cells involved in the regeneration of trophic ulcers has not been studied enough. Our aim was to study the effect of platelet lysate and platelet-poor plasma from patients with trophic Research Institute of Clinical and Experimental Lymphology — Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences; 2E. N. Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk, Russia, Address for correspondence: [email protected]. A. P. Lykov 1
ulcers of different genesis on the proliferation, migration, apoptosis, and cell cycle of human dermal fibroblasts, mesenchymal stem cells, and EA.hy 926 endothelial cells in vitro.
MATERIALS AND METHODS The study was conducted in strict adherence to the principles of the Helsinki Declaration; informed consent was obtained from all participants. The research included 7 patients with trophic ulcers with type 2 diabetes mellitus (group 1), and 7 patients with trophic ulcers of venous etiology (group 2). Platelet lysate (PL) and platelet-poor plasma (PPP) were obtained as described previously [11]. Dermal fibroblast (DF) were derived fr
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