Experimental Measurement of Particle Velocity in a High Reynolds Micro-channel Flow
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Original Article
Experimental Measurement of Particle Velocity in a High Reynolds Micro-channel Flow Jongin Choi & Sunghwan Jung
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Received: 6 February, 2020 / Accepted: 14 June, 2020 / Published online: 27 August, 2020 â’¸The Korean BioChip Society and Springer 2020
Abstract The present work is to provide experimental measurements of particle velocity in a microchannel flow at the Reynolds number of 10 and their respective particle positions in the channel where the velocities were measured. The particle position measurements which includes their depths were realized without an intricate optical set-up by controlling the particle path with multiple flow rates. The experimental measurements of particle velocity combined with the position measurements were subsequently used to confirm accuracy of particle velocities numerically estimated by a flow simulation exclusive of particle migration. The numerical estimates and experimental measurements of particle velocity agree with a difference of only less than 5%. The agreement was also supported by a detailed quantification. Keywords: Depth, High Reynolds number particle, Microchannel flow, Particle velocity, Position
Introduction Micro-flow cytometry using microchannel platforms has been explored with the view of developing affordable and suitably sized implements for point of care testing1-4. Optical characterization of particles in the micro-flow cytometer is performed by focusing particles in three dimension (3D) in the microchannel flow. Thus, it is important to quantitatively inspect Department of Mechanical Engineering, Dankook University, 152 Jukjeon-ro Suji-gu, Yongin-si 16890, Korea *Correspondence and requests for materials should be addressed to S.H. Jung ( [email protected])
the 3D focusing performance for optimal flow cytometer operation. Quantitative inspection of the 3D focusing performance requires knowledge of depth position and lateral position of moving particles. For the depth detection, which is challenging to achieve, several expensive optical set-ups were designed. However, they were only verified at the channel flow velocities within the range of ~ 1 mm/sec5-12 where the flow cytometry throughput could be limited. As an alternative to meet the high velocity flow demand, particle velocity measurements can be adopted to detect the depth of particles13. In the velocity-based approach, two-dimensional imaging of moving particles are recorded using a high-speed camera assembled with an optical microscope to identify the particle lateral position and estimate the particle velocity. Then, the particle velocity profile at the particle lateral position is constructed across the channel cross section using numerical computations. From the velocity profile, the particle depth is located where the experimentally obtained and numerically estimated velocities coincide as shown in Figure 1. The velocity-based depth detection technique is simple and inexpensive to operate as it does without expensive optical set-ups and complicated processing. The ease o
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