Expression of cellulose synthase-like genes in two phenotypically distinct flax ( Linum usitatissimum L.) subspecies
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RESEARCH ARTICLE
Expression of cellulose synthase-like genes in two phenotypically distinct flax (Linum usitatissimum L.) subspecies Dmitry Galinousky . Tsimafei Padvitski . Natalia Mokshina . Oleg Gorshkov . Lubov Khotyleva . Tatyana Gorshkova . Alexandr Kilchevsky
Received: 10 October 2019 / Accepted: 13 April 2020 Ó Springer Nature B.V. 2020
Abstract Cellulose synthase-like (CSL) genes encode glycosyltransferases thought to be involved in the biosynthesis of cell wall non-cellulosic polysaccharides; however, the exact function is not established yet for many of them. We performed a bioinformatics search of CSL genes in the flax genome, their phylogenetic analysis and evaluated expression of CSL genes in the course of plant development and in various plant parts. Quantitative RT-PCR was used to examine expression of CSL genes in several fiber-containing samples of two flax subspecies at seedlings, rapid growth and budding Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-00943-2) contains supplementary material, which is available to authorized users. D. Galinousky (&) L. Khotyleva A. Kilchevsky Institute of Genetics and Cytology, Akademichnaya, 27, 220072 Minsk, Belarus e-mail: [email protected] D. Galinousky Belarusian State University, Nezalezhnasci, 4, 220030 Minsk, Belarus T. Padvitski University of Cologne, CECAD, Joseph-Stelzmann-Str., 26, 50931 Cologne, Germany N. Mokshina O. Gorshkov T. Gorshkova Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky st., 2/31, P.O. 30, 420111 Kazan, Russia
stages. One of the studied subspecies is fiber flax (cultivar Blakit) that was developed to produce highquality fiber and another one is dehiscent flax that is not used for fiber production. Our study has revealed that the LusCSLD3 and LusCSLG3.2/3.3 genes were highly expressed in all studied samples and their expression was comparable with the expression of cellulose synthase genes involved in secondary cell wall development (LusCESA4 and LusCESA7-B) at rapid growth and budding stages. LusCSLB4 gene had a strong expression in seedling samples only. We have found that LusCSL gene expression profiles from the two phenotypically distinct flax subspecies are more different at the seedling stage than at the later developmental stages—expression of LusCSL was higher in fiber flax Blakit at the seedling stage as compared to dehiscent flax. Analysis of data from RNA-Seq experiments performed on various parts of flax plants revealed that LusCSLG with unknown substrate activity could be a specific glycosyltransferase involved in the synthesis of a non-cellulosic polysaccharide composing the fiber cell wall. Our findings lay the foundation for the understanding of the fiber cell-wall biogenesis and suggest characteristic features of this process in the cultivated flax. Keywords Fiber Cellulose synthase-like (CSL) genes Flax Quantitative reverse transcription PCR RNA
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