Fire as a novel technique to stimulate adventitious shoots in the laboratory
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RESEARCH NOTE
Fire as a novel technique to stimulate adventitious shoots in the laboratory Bárbara Paula dos Santos Borges1 · Andressa Priscila Piancó Santos Lima1 · Alone Lima‑Brito1 · José Raniere Ferreira de Santana1 · Abel Augusto Conceição1,2 Received: 28 May 2020 / Accepted: 12 September 2020 © Springer Nature B.V. 2020
Abstract Fire-stimulated flowering and fire-stimulated resprouting are associated traits common in plants that evolved in fire-prone habitats, e.g., Vellozia pyrantha, an iconic plant that has economic potential and is endemic to a fully protected area. By combining a natural phenomenon that drives demography and in situ evolution, we tested if fire can be used as a novel technique for plant multiplication through vegetative bud activation in V. pyrantha. The basal parts of microplants cultivated for 60 days (5 to 6 cm tall) were exposed to a flame using a Bunsen burner for five periods controlled by a timer (2, 4, 6, 8, and 10 s) and compared to unburned plants (control). After 30 days, the number of new shoots was counted, and the length of the longest shoot produced per individual was measured. Adventitious shoot development started 7 days after the fire incidence. The number of shoots per individual and length of the longest shoot produced were significantly higher in the 6- to 10-s treatments. Fire was an efficient and cheap way to induce adventitious shoot formation. This new method could also be applied to other resprouter species from fire-prone environments in the world. Key message By combining a natural phenomenon that drives evolution with the bud stimulus expected, we found fire as an efficient and cheap way to induce buds. Keywords Bud induction · Fire-prone environment · Fire-stimulated resprouting · In vitro propagation · Plant multiplication experiment · Vellozia pyrantha Bud induction is a subject of great interest and applied in many areas, such as agriculture, horticulture and ecological restoration, because it allows the vegetative reproduction of plants. In the laboratory, temperature, air humidity, photoperiod, and nutrient and water availability are essential factors controlled during different steps of plant propagation Communicated by Amita Bhattacharya. * Abel Augusto Conceição [email protected]; [email protected] 1
Programa de Pós‑Graduação em Recursos Genético Vegetais, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, Feira de Santana, Bahia 44036‑900, Brazil
Programa de Pós‑Graduação em Botânica, Universidade Estadual de Feira de Santana, Av. Transnordestina s/n, Feira de Santana, Bahia 44036‑900, Brazil
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(Pati et al. 2006; Hussain et al. 2012; Viehmannova et al. 2016; Regalado et al. 2020). In nature, these abiotic factors also affect the plant developmental stages (Piao et al. 2019) but are prone to sudden alterations caused by disturbance events, such as fire, an important driver of plant evolution in fire-prone environments in the world (Whelan 1995; Lamont and Downes 2011). Vellozia pyrantha A.A.Conc. occurs in a fire-prone ecosys
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