First evidence for allotriploid hybrids between Juniperus thurifera and J. sabina in a sympatric area in the French Alps
- PDF / 1,895,785 Bytes
- 17 Pages / 595.276 x 790.866 pts Page_size
- 10 Downloads / 162 Views
RESEARCH PAPER
First evidence for allotriploid hybrids between Juniperus thurifera and J. sabina in a sympatric area in the French Alps Perla Farhat 1,2 & Najat Takvorian 2,5 & Maria Avramidou 2 & Luc Garraud 3 & Robert P. Adams 4 & Sonja Siljak-Yakovlev 2 & Magda Bou Dagher Kharrat 1 & Thierry Robert 2,5 Received: 16 November 2019 / Accepted: 28 May 2020 # INRAE and Springer-Verlag France SAS, part of Springer Nature 2020
Abstract & Key message Discovery of the first case of allotriploid juniper in a wild population in the French Alps where the parental species occurs in sympatry. & Context Interspecific hybridization and polyploidy are important evolutionary phenomena in vascular plants. Natural hybridization between species living in sympatry can sometimes occur. Less frequent are successful hybridizations between species having different ploidy levels. At Saint Crépin location (French Alps), where sympatry between the tetraploid Juniperus thurifera and the diploid Juniperus sabina occurs, three individuals with an atypical morphology have been observed. & Aims Prospect interspecific hybrids and interspecific genetic introgression occurrence. & Methods Flow cytometry was employed to screen ploidy levels. Four chloroplast markers, nrDNA (ITS), and AFLP markers were used to unravel hybridization and potential introgression events. Variability of pollen size and morphology was assessed to have a first insight on the regularity of microsporogenesis in hybrids. & Results The three putative hybrids were shown to be triploids. Molecular data demonstrated that these individuals were hybrids originated from a cross between J. sabina and J. thurifera and suggested that a backcross at least with J. thurifera is possible. Male triploid hybrids produced heterogeneous pollen and displayed evidence of irregularity in the microsporogenesis. & Conclusion This study sheds light on a rare case of hybridization in a natural sympatric population of two Juniperus species with different ploidy levels. This mechanism might have been an important driver for the evolution and diversification of this coniferous genus. Keywords Juniperus . Polyploidy . Interspecific hybridization . Pollen abnormality . AFLP . Saint Crépin
Handling Editor: Bruno Fady * Perla Farhat [email protected] Najat Takvorian [email protected]
Thierry Robert [email protected] 1
Laboratoire Biodiversité et Génomique Fonctionnelle, Faculté des Sciences, Université Saint-Joseph, Campus Sciences et Technologies, Mar Roukos, Mkalles, BP: 1514 Riad el Solh, Beirut 1107 2050, Lebanon
2
Ecologie Systématique Evolution, Université Paris-Saclay, CNRS, AgroParisTech, 91400 Orsay, France
3
Conservatoire Botanique National Alpin, Domaine de Charance, 05000 Gap, France
Sonja Siljak-Yakovlev [email protected]
4
Biology Department, Baylor University, Box 97388, Waco, TX 76798, USA
Magda Bou Dagher Kharrat [email protected]
5
Biology Department, Sorbonne Université, 4 place Jussieu, 75005 Paris, France
Maria Avramidou maria.avrami
Data Loading...
