First trimester screening for trisomy 21 in gestational week 8-10 by ADAM12-S as a maternal serum marker
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RESEARCH
Open Access
First trimester screening for trisomy 21 in gestational week 8-10 by ADAM12-S as a maternal serum marker Niels Tørring1*, Susan Ball2, Dave Wright2, Gaïané Sarkissian3, Marie Guitton3, Bruno Darbouret3
Abstract Background: A disintegrin and metalloprotease 12 (ADAM12-S) has previously been reported to be significantly reduced in maternal serum from women with fetal aneuploidy early in the first trimester and to significantly improve the quality of risk assessment for fetal trisomy 21 in prenatal screening. The aim of this study was to determine whether ADAM12-S is a useful serum marker for fetal trisomy 21 using the mixture model. Method: In this case control study ADAM12-S was measured by KRYPTOR ADAM12-S immunoassay in maternal serum from gestational weeks 8 to 11 in 46 samples of fetal trisomy 21 and in 645 controls. Comparison of sensitivity and specificity of first trimester screening for fetal trisomy 21 with or without ADAM12-S included in the risk assessment using the mixture model. Results: The concentration of ADAM12-S increased from week 8 to 11 and was negatively correlated with maternal weight. Log MoM ADAM12-S was positively correlated with log MoM PAPP-A (r = 0.39, P < 0.001), and with log MoM free beta hCG (r = 0.21, P < 0.001). The median ADAM12-S MoM in cases of fetal trisomy 21 in gestational week 8 was 0.66 increasing to approx. 0.9 MoM in week 9 and 10. The use of ADAM12-S along with biochemical markers from the combined test (PAPP-A, free beta hCG) with or without nuchal translucency measurement did not affect the detection rate or false positive rate of fetal aneuploidy as compared to routine screening using PAPP-A and free b-hCG with or without nuchal translucency. Conclusion: The data show moderately decreased levels of ADAM12-S in cases of fetal aneuploidy in gestational weeks 8-11. However, including ADAM12-S in the routine risk does not improve the performance of first trimester screening for fetal trisomy 21.
Background The soluble form of A disintegrin and metalloprotease 12 (ADAM12-S) with suggested proteolytic activity on Insulin-like growth factor binding protein (IGFBP) - 3 and 5 and the epidermal growth factor ligands EGF, Betacellulin and HB-EGF [1], is synthesized by the placenta syncytiotrophoblasts [2,3] and is present in high concentrations in maternal serum from early first trimester [4-6]. Since ADAM12-S was first reported to be significantly reduced in maternal serum from women with fetal trisomy 21 [4] much attention has been given to investigating the utility of ADAM12-S as a prenatal * Correspondence: [email protected] 1 Department of Clinical Biochemistry, Aarhus University Hospital - Skejby, Aarhus, Denmark Full list of author information is available at the end of the article
marker for fetal aneulpoidy in first and second trimester [4-9], fetal pre-eclampsia [10,11] and intra uterine growth restriction [12]. The discriminatory efficiency of ADAM12-S as a marker for fetal aneuploidy in first trimester has been inconclusive. Reported median multipl
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