Fluorescent Methods for Molecular Motors
This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of m
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Christopher P. Toseland Natalia Fili Editors
Fluorescent Methods for Molecular Motors
Experientia Supplementum Volume 105
For further volumes: http://www.springer.com/series/4822
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Christopher P. Toseland • Natalia Fili Editors
Fluorescent Methods for Molecular Motors
Editors Christopher P. Toseland Chromosome Organisation and Dynamics Max-Planck Institute of Biochemistry Martinsried Germany
Natalia Fili Department of Cellular Physiology Ludwig-Maximilians-Universita¨t Mu¨nchen Munich Germany
ISSN 1023-294X ISBN 978-3-0348-0855-2 ISBN 978-3-0348-0856-9 (eBook) DOI 10.1007/978-3-0348-0856-9 Springer Basel Heidelberg Dordrecht New York London Library of Congress Control Number: 2014947103 © Springer Basel 2014 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. Exempted from this legal reservation are brief excerpts in connection with reviews or scholarly analysis or material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Duplication of this publication or parts thereof is permitted only under the provisions of the Copyright Law of the Publisher’s location, in its current version, and permission for use must always be obtained from Springer. Permissions for use may be obtained through RightsLink at the Copyright Clearance Center. Violations are liable to prosecution under the respective Copyright Law. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. While the advice and information in this book are believed to be true and accurate at the date of publication, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
For Thalia. . .You remind us of how much we can learn every day.
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Preface
The term “Fluorescence” was initially coined by George Gabriel Stokes in 1852, but the phenomenon had already been recorded for over 300 years. Our knowledge of fluorescence expanded through the pioneering work of Perrin, Jablonski and Fo¨rster and Weber. This work coincided with the synthesis of organic dyes—the fluorophores. Our ways to manipulate fluorophores have grown to su
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