Food size and cGMP affects feeding behaviour in Pristionchus pacificus
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POSTER PRESENTATION
Open Access
Food size and cGMP affects feeding behaviour in Pristionchus pacificus Silvina Kroetz1*, Jagan Srinivasan2, Paul Sternberg2, Ray L Hong1 From International Society of Sports Nutrition: 8th Annual ISSN Conference and Expo Las Vegas, NV, USA. 24-25 June 2011 Background Animals evolved different locomotory behaviors in order to find food in their environment. I studied the food seeking locomotion and pharyngeal pumping of nematodes Pristionchus pacificus on various food sources. Methods For this study I used P. pacificus PS312, and the mutants Ppa-egl-4, which is a null mutation in the cGMP dependent protein kinase, and Ppa-obi-1, which is an oriental beetle pheromone insensitive mutant, and the double mutant Ppa-egl-4;obi-1. I tested these strains on plates containing no food and on E.coli OP50, HB101, Caulobacter crescentus (NA1000) and Bacillus subtilis. I analyzed locomotory behavior using an automated tracking system, and I obtained pharyngeal pumping data by visually counting with a microscope at 80X magnification. Results I observed that locomotion of the strains differed on plates with no food and plates with food. On plates with no food, P. pacificus PS312 displayed a higher reversal rate compared to the Ppa-obi-1 strain. The double mutant egl-;obi-1 displayed similar locomotion patterns to Ppa-obi-1 on HB101. Furthermore, when I compared PS312 pharyngeal pumping rates on and off food on two different size bacteria E. coli and C. crescentus, results showed a significant increased rate on PS312 on C. crescentus, which was the smaller bacteria. Conclusion My results indicated that Ppa-obi-1 may act in either a parallel pathway, or upstream of Ppa-egl-4. PS312 raised * Correspondence: [email protected] 1 Department of Biology, California State University Northridge, USA Full list of author information is available at the end of the article
on C. crescentus (NA1000) for 3 generations retained memory of the food experience regardless of whether they were removed from food or placed back on NA1000 as food. Increasing bacterial size using mutant C. crescentus strains seem to further decrease pumping rates off food. My data suggest strong roles for food sizes and cGMP sensing proteins in maintaining feeding patterns in P. pacificus. Author details 1 Department of Biology, California State University Northridge, USA. 2Division of Biology, California Institute of Technology, Pasadena, USA. Published: 7 November 2011
doi:10.1186/1550-2783-8-S1-P26 Cite this article as: Kroetz et al.: Food size and cGMP affects feeding behaviour in Pristionchus pacificus. Journal of the International Society of Sports Nutrition 2011 8(Suppl 1):P26.
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