FpCzf14 is a putative C 2 H 2 transcription factor regulating conidiation in Fusarium pseudograminearum
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Phytopathology Research
RESEARCH
Open Access
FpCzf14 is a putative C2H2 transcription factor regulating conidiation in Fusarium pseudograminearum Linlin Chen1,2, Jingya Zhao1, Huiqing Xia1, Yuming Ma1, Yankun Liu1, Mengya Peng1, Xiaoping Xing1, Bingjian Sun1, Yan Shi1 and Honglian Li1,2*
Abstract C2H2 zinc finger transcription factors such as FlbC and Msn2, have broad regulatory roles in fungal growth and conidiation. In the present study, we cloned and characterized a C2H2 zinc finger transcription factor gene, FpCzf14, in the wheat pathogen Fusarium pseudograminearum. FpCzf14 was localized to the nuclei. The expression of FpCzf14 was significantly upregulated in conidia, suggesting that FpCzf14 might contribute to conidiation. Further analysis of the fpczf14-deleted mutant (Δfpczf14) demonstrated that it exhibited defect in conidiation, and this defect was restored in the complemented strain Δfpczf14-C expressing FpCzf14, demonstrating that FpCzf14 was essential for conidiation. Moreover, FpCzf14 was required for mycelial growth and pathogenicity of F. pseudograminearum. Microscopic observation results showed that Δfpczf14 produced only very few penetration pegs and invasive hyphae inside host tissues compared with WT and Δfpczf14-C. Additionally, results of reverse transcription quantitative PCR (RT-qPCR) showed that FpCzf14 regulated expression of several conidiation-related genes in F. pseudograminearum. In conclusion, FpCzf14, as a core regulatory gene in conidiation, provides new insights into the mechanism of conidiation in F. pseudograminearum. Keywords: Fusarium pseudograminearum, Transcription factors, FpCZF14, Conidiation, Pathogenicity
Background Fusarium pseudograminearum, commonly found in soil and various decaying plant materials, is an important pathogen causing Fusarium crown rot (FCR) and Fusarium head blight (FHB) of wheat and barley (Kazan and Gardiner 2018; Zhou et al. 2019). Infection with F. pseudograminearum compromises grain yield and quality, and more importantly, F. pseudograminearum is a mycotoxinproducing species like some other Fusarium spp. that seriously threaten human health through the production of toxins (Tunali et al. 2012; Kazan and Gardiner 2018). In recent years, with the development and application of molecular biological techniques in plant pathogens, the * Correspondence: [email protected] 1 College of Plant Protection, Henan Agricultural University, Zhengzhou 450000, China 2 National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450000, China
functions of a few genes in F. pseudograminearum genome have been elucidated. For example, heat shock protein 70 (FpLhs1) is involved in protein secretion (Chen et al. 2019); Fdb3 and Tri5 function in mycotoxin production (Kettle et al. 2016; Powell et al. 2017); and FpAda1 and FpDep1 are necessary for growth and pathogenesis of F. pseudograminearum (Chen et al. 2020; Zhang et al. 2020). However, the functions of genes encoding C2H2 zinc finger (CZF) transcription factors (TFs) are unknown in F. pseudograminearum
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