Genomic information on Stenotrophomonas maltophilia ST264 isolated from a cystic fibrosis pediatric patient in Brazil
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CLINICAL MICROBIOLOGY - SHORT COMMUNICATION
Genomic information on Stenotrophomonas maltophilia ST264 isolated from a cystic fibrosis pediatric patient in Brazil Fabrina Seger Braga 1 & Ana Paula D’Allincourt Carvalho Assef 2 & Robson Souza Leão 1 & Rodolpho Mattos Albano 3 & Elizabeth Andrade Marques 1 Received: 11 September 2019 / Accepted: 11 December 2019 # Sociedade Brasileira de Microbiologia 2019
Abstract Stenotrophomonas maltophilia is one of the Gram-negative bacilli most frequently found in the airways of cystic fibrosis patients. This opportunistic pathogen is intrinsically multidrug-resistant, and therefore, its treatment presents a challenge. The genetic characterization of S. maltophilia is largely unknown, especially from those strains that colonize/infect the airways of cystic fibrosis patients. This work reports the draft genome sequences of three S. maltophilia isolates recovered from the sputum of a cystic fibrosis pediatric patient in Southeast Brazil. Several resistance- and virulence-related genes were detected. Furthermore, one intact phage and one incomplete prophage region were also identified in all strains. Multilocus sequence typing showed that all strains belonged to a new sequence type (ST264). Interestingly, all S. maltophilia strains were genetically identical, showing persistence for at least 16 months. To our knowledge, this is the first report of S. maltophilia draft genome sequences obtained from a cystic fibrosis pediatric patient in Brazil. Keywords Cystic fibrosis . Genome sequence . Resistance . Virulence . Stenotrophomonas maltophilia
Stenotrophomonas maltophilia is a Gram-negative bacillus that has emerged as an important opportunistic pathogen in cystic fibrosis (CF) patients. However, the consequences of its presence in this group of patients remain controversial [1]. A recent study associated this micro-organism with an acceleration in lung function decline, particularly in long-term colonized/infected patients [2]. S. maltophilia is intrinsically resistant to multiple antibiotic classes and has been described
Responsible Editor: Mara Correa Lelles Nogueira * Elizabeth Andrade Marques [email protected] 1
Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida Boulevard 28 de Setembro, 87, fundos, 3° andar, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
2
Laboratório de Pesquisa em Infecção Hospitalar (LAPIH), Instituto Oswaldo Cruz (FIOCRUZ), Avenida Brasil, 4.365, Pavilhão Rocha Lima - Sala 314, Manguinhos, Rio de Janeiro, RJ 21040-360, Brazil
3
Departamento de Bioquímica, Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida Boulevard 28 de Setembro, 87, 4°andar, Vila Isabel, Rio de Janeiro, RJ 20551-030, Brazil
in 10 to 18% of CF patients. Knowledge about the mechanisms this pathogen uses to colonize and persist in the airways of CF patients is imperative to inform and improve the treatment [3, 4]. Genomic features are largely underst
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