Glucose, insulin, and cortisol concentrations and glucose tolerance test in Holstein cows with inactive ovaries
- PDF / 849,626 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 101 Downloads / 242 Views
REGULAR ARTICLES
Glucose, insulin, and cortisol concentrations and glucose tolerance test in Holstein cows with inactive ovaries Reza Azarbayejani 1 & Majid Mohammadsadegh 2 Received: 1 June 2020 / Accepted: 15 October 2020 # Springer Nature B.V. 2020
Abstract A total of 60 healthy (the control group) and 30 anestrous Holstein cows with inactive ovaries (the case group) from three dairy farms around Tehran were randomly selected and diagnosed to determine the concentrations of glucose, insulin, and cortisol, and the rate of the insulin resistance disorder in them. Primarily, serum samples were obtained from all animals, and the concentrations of glucose, insulin, and cortisol were measured in a laboratory. Thereupon, the glucose tolerance test (GTT) was implemented. Before the implementation of the GTT, the median of serum glucose levels (62 mg/dl), insulin (7.16 μIU/ml), and cortisol (2.5 μg/dl) was estimated in healthy cows. The median of serum glucose levels (68.5 mg/dl, p = 0.00009) was higher, and serum insulin (6.1 μIU/ml, p = 0.025) and cortisol (1.1 μg/dl, p = 0.00029) levels were lesser in the anestrous cows when estimated simultaneously. After the implementation of GTT, the median of serum glucose concentrations in one (120 mg/dl), two (73.5 mg/dl), and three (63.5 mg/dl) hours after the glucose injection was estimated in healthy cows. The findings also showed that the median of serum glucose levels was lesser in one (100 mg/dl, p = 0.015), similar in two (77.5 mg/dl, p = 0.9), and higher in three (70 mg/dl, p = 0.012) hours after the glucose injection in anestrous cows. In conclusion, higher serum glucose, lesser insulin and cortisol concentrations, and the different correlation between serum glucose and insulin levels before the GTT were detected in anestrous cows. The pattern of glucose changes also differed in anestrous cows after the GTT. Keywords Dairy cows . GTT . Glucose . Inactive ovary . Insulin resistance
Introduction A prolonged period of postpartum anestrus due to inactive ovaries or anovulatory follicles is considered the most current factor producing infertility in cows after uterine infection, repeat breeder, and m astitis (Bartlett et al. 1987; Abeygunawarden and Dematawew 2004). About 20% of dairy cows in anestrus by the start of breeding programs (Rhodes et al. 2003) or by 63 days after calving (Santos et al. 2004) were involved in the inactive ovary. On the other hand, some other cows show ovarian rebound and estrous cycles at the normal time after calving and maybe even inseminated, and later relapse back into an inactive ovary (Peter * Majid Mohammadsadegh [email protected] 1
Internal Medicine, Faculty of Veterinary Medicine, Sciences and researches Branch, Islamic Azad University, Tehran, Iran
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
et al. 2009; Noakes et al. 2019); therefore, the condition by turn may increase calving to conception intervals, and calving intervals (Noakes et al. 2019; Mohamma
Data Loading...
