Granulosa cell-conditioned medium enhances steroidogenic competence of buffalo ( Bubalus bubalis ) theca cells
- PDF / 1,556,149 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 3 Downloads / 174 Views
Granulosa cell-conditioned medium enhances steroidogenic competence of buffalo (Bubalus bubalis) theca cells Jun Zhang 1
&
Yanfei Deng 1
&
Jianchun Xu 1 & Xiaofen Yang 1 & Haoxin Wang 1 & Deshun Shi 1 & Fenghua Lu 1
Received: 22 May 2020 / Accepted: 11 September 2020 / Editor: Tetsuji Okamoto # The Society for In Vitro Biology 2020
Abstract Granulosa cells (GCs) and theca cells (TCs) are the main components of follicles, and the interactions between GCs and TCs play a significant role in steroidogenesis, follicular growth, and atresia. However, the effects of GCs in the form of conditioned medium on steroidogenesis in buffalo TCs remain unclear. In the present study, the impacts of GC-conditioned medium (GCCM) on androgen synthesis in buffalo TCs were examined. The results showed that GCCM collected at 48 h promoted both the expression levels of androgen synthesis-related genes (CYP11A1, CYP17A1, 3β-HSD, and Star) and the secretion levels of testosterone in TCs. The treatment time of 48 h in GCCM improved both the expression levels of androgen synthesis-related genes (CYP11A1, CYP17A1, 3β-HSD, and Star) and the secretion levels of testosterone in TCs. Furthermore, GCCM that was collected at 48 h and applied to TCs for 48 h (48 h and 48 h) promoted the sensitivity of buffalo TCs to LH. This study indicated that GCCM (48 h and 48 h) enhanced the steroidogenic competence of TCs mainly through facilitating the responsiveness of TCs to LH in buffalo. This study provides a basis for further exploration of interactions between GCs and TCs for steroidogenesis in the ovary. Keywords Buffalo . Granulosa cells . Conditioned medium . Theca cells . Steroidogenesis
Introduction Oocytes, granulosa cells (GCs), and theca cells (TCs) are the main components of follicles, and the interactions among them play an essential role in steroidogenesis, follicular development, and atresia (Orisaka et al. 2009). Oocyte-granulosa cell communications, largely carried out by gap junctions, are necessary for follicular growth and oocyte maturation in vivo. Some paracrine factors such as natriuretic peptide type C (CNP), brain-derived neurotrophic factor (BDNF), and insulin-like growth factors-I (IGF-I) are specifically secreted by GCs (Zhang et al. 2010; Oberlender et al. 2013; Zhao et al. 2019; Rouhollahi Varnosfaderani et al. 2020). These factors regulate follicular development and contribute to the modulation of oocyte maturation. However, unlike that of GCs and oocytes, the interactions between GCs and TCs in follicles have not been extensively explored. Therefore, it is important
* Fenghua Lu [email protected] 1
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China
to understand more about the relationship between GCs and TCs in follicular development. It is generally accepted that TCs provide androgen for estrogen biosynthesis in GCs, which stimulates the early follicular development (Wang et al. 2001; Stocco et al. 2007; Tajima et al. 2007; Gervasio et al. 2014).
Data Loading...
