Guidelines for Molecular Analysis in Archive Tissues
A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to th
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Giorgio Stanta (Editor)
Guidelines for Molecular Analysis in Archive Tissues
Editor Prof. Dr. Giorgio Stanta Department of Medical Sciences University of Trieste Cattinara Hospital Strada di Fiume 447 Trieste Italy [email protected]
ISBN 978-3-642-17889-4 e-ISBN 978-3-642-17890-0 DOI 10.1007/978-3-642-17890-0 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2011925943 © Springer-Verlag Berlin Heidelberg 2011 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publishers cannot guarantee the accuracy of any information about dosage and application contained in this book. In every individual case the user must check such information by consulting the relevant literature. Cover design: eStudioCalamar, Figueres/Berlin Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
These guidelines are devoted to disseminate molecular methods that can be used to analyze DNA, RNA, and proteins in formalin-fixed and paraffin-embedded (FFPE) tissues. We have called them guidelines because this is the first method book that is entirely dedicated to archive tissues. They are addressed to pathologists, biologists, and biotechnologists who do research in FFPE tissues. The protocols presented in these guidelines derive from the experience of the laboratories participating in the European project “Archive tissues: improving molecular medicine research and clinical practice” – IMPACTS (www.impactsnetwork.eu). The 20 participants, from 11 European countries, are some of the most experienced groups in molecular analysis of fixed and paraffin-embedded human tissues. Among them are some of the groups that developed this type of molecular analysis for the first time. These guidelines are mostly dedicated to the homemade protocols that were developed, tested, and even validated by multicentric analyses performed by the IMPACTS groups. Experience in basic molecular methods is necessary to develop molecular pathology activities in human tissues. This allows research without too many technical limitations. In this way the researcher is also capable to better evaluate commercial kits and validate them. Also commercial kits are reported i
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