High-resolution solid state NMR experiments for the characterization of calcium phosphate biomaterials and biominerals
- PDF / 701,344 Bytes
- 14 Pages / 584.957 x 782.986 pts Page_size
- 72 Downloads / 219 Views
ck Fayon CEMHTI, CNRS UPR 3079, 45071 Orléans cedex 2, France
Sara Laurencin Dalicieux, Isabelle Gennero, and Jean-Pierre Salles INSERM Unité 563 (Centre de Physiopathologie de Toulouse Purpan), Université Paul-Sabatier, Hôpital Purpan, CHU de Toulouse, 31059 Toulouse cedex 9, France
Andrew P. Howes, Ray Dupree, John V. Hanna, and Mark E. Smith Department of Physics, University of Warwick, CV4 7AL Coventry, UK
Francesco Mauri Laboratoire de Minéralogie Cristallographie UMR CNRS 7590, UPMC Université Paris 06, France
Gilles Guerrero, P. Hubert Mutin, and Danielle Laurencina) Institut Charles Gerhardt de Montpellier, UMR 5253, CNRS UM2 UM1 ENSCM, CC 1701 Université de Montpellier 2, 34095 Montpellier cedex 5, France (Received 10 April 2011; accepted 22 July 2011)
Calcium phosphates form a vast family of biominerals, which have attracted much attention in fields like biology, medicine, and materials science, to name a few. Solid state Nuclear Magnetic Resonance (NMR) is one of the few techniques capable of providing information about their structure at the atomic level. Here, examples of recent advances of solid state NMR techniques are given to demonstrate their suitability to characterize in detail synthetic and biological calcium phosphates. Examples of high-resolution 31P, 1H (and 17O), solid state NMR experiments of a 17O-enriched monocalcium phosphate monohydrate-monetite mixture and of a mouse tooth are presented. In both cases, the advantage of performing fast Magic Angle Spinning NMR experiments at high magnetic fields is emphasized, notably because it allows very small volumes of sample to be analyzed.
I. INTRODUCTION
Calcium phosphates form a vast family of biominerals, which are present in a large number of biological tissues.1–5 Hydroxyapatite (HA) is the most studied calcium phosphate phase because it is the main mineral component of bone and teeth. Its composition differs from that of stoichiometric HA [Ca10(PO4)6(OH)2] due to the presence of several ionic substitutions in the HA lattice, such as CO32 , HPO42 , Na1, and Mg21. Brushite (dicalcium phosphate dihydrate, CaHPO4.2H2O) and octacalciumphosphate [OCP, Ca8(HPO4)2(PO4)4.5H2O] phases have been observed in pathological calcifications like dental calculi and urinary stones.2 Substituted b-Ca3(PO4)2 (b-TCP) can also be present in some forms of dental calculi and salivary stones, while calcium pyrophosphate
a)
Address all correspondence to this author. e-mail: [email protected] This paper has been selected as an Invited Feature Paper. DOI: 10.1557/jmr.2011.250 J. Mater. Res., Vol. 26, No. 18, Sep 28, 2011
http://journals.cambridge.org
Downloaded: 09 Oct 2014
dihydrate (Ca2P2O7.2H2O) crystals can form in connective tissues and joints, leading to diseases like pseudo-gout (also referred to as Calcium Pyrophosphate Deposition Disease) and acute arthritis. In addition to these crystalline calcium phosphates, another important phase is amorphous calcium phosphate (ACP, CaxHy(PO4)z.nH2O). ACP is generally unstable in physiological cond
Data Loading...
