Histology Protocols
With much of what we know about the pathogenesis of disease coming from the systematic and careful study of histological material, histology has proven to be an incredibly valuable area of study. In Histology Protocols, expert researchers in the field con
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Detection, systems, biotin Bouin’s fixative, see Fixative, Bouin’s
C Caliper..................................................................... 212–214 Cavalieri’s method .......................................................... 223 Cellular retinol-binding protein-1 (CRBP-1) ................. 65 Chromogenic substrates, see Detection, substrates Collagen..................6, 48, 69, 131–132, 134, 136–137, 139 Colloidal gold, see Detection, systems, colloidal gold Colocalization ................................................. 174, 184, 189 Color shift correction.............................................. 188–189 Confocal microscopy, see Microscopy, confocal Controls, specificity of staining .................................. 68–69 Cross-linking, see Fixative, cross-linking Cryofixation .......................................................... 46, 49, 93 Cultured cells .............................................. 43–56, 167, 180 Cytoskeleton ............................................................... 43–56
D Deconvolution .......................................... 44, 177, 184–191 Delesse’s principle........................................................... 219 Depth of field.................................................. 183, 193–210 Detection, substrates diaminobenzidine (DAB) ..................................... 62, 71 new fuchsin ........................................................... 61, 69
Detection, systems alkaline phosphatase ............................................. 59–71 biotin................................................................... 97, 166 colloidal gold..................................................... 107, 110 digoxigenin........................................................ 116, 124 tyramide .................................................................... 127 Digoxigenin, see Detection, systems, digoxigenin Disector........................................................... 219, 222–223 DNA fragmentation ....................................................... 162 Double labeling............................................................... 169 Duplex in situ hybridization (dISH) ...................... 115–128 Dynamic range................................................ 190, 193–210 Dynamic range increase (DRI)...... 194–196, 199–202, 204, 206–207, 209–210
E Elastic system.................................................................. 131 Electron microscopy, see Microscopy, Transmission electron Embedding frozen ........................................................ 13–14, 33, 34 paraffin .................................................................. 14, 34 Embryos .............................................................. 4, 141–149 Endogenous enzyme activity ............................................ 66 Extracellular matrix............................. 4, 6, 43–56, 131–139
F Fibrosis.......................................................... 6, 38, 132, 136 Fixation immersion ....................................... 4, 11, 144–147, 149
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