hsa_circ_0006916 promotes hepatocellular carcinoma progression by activating the miR-337-3p/STAT3 axis
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Cellular & Molecular Biology Letters
Open Access
RESEARCH
hsa_circ_0006916 promotes hepatocellular carcinoma progression by activating the miR‑337‑3p/STAT3 axis Xiao‑Yong Zhou*, Hui Yang, Yang‑Qiu Bai, Xiu‑Ling Li, Shuang‑Yin Han and Bing‑Xi Zhou *Correspondence: [email protected] Department of Gastroenterology and Hepatology, Henan Provincial People’s Hospital, People’s Hospital of Zhengzhou University, No. 7 Weiwu Road, Jinshui District, Zhengzhou 450003, Henan, China
Abstract Background: Circular RNAs (circRNAs) are thought to be involved in the development of various malignancies. The expression and function of hsa_circ_0006916, a newly identified circRNA, in hepatocellular carcinoma remain unclear. Methods: Quantitative RT-PCR was used to detect hsa_circ_0006916 in hepatocellular carcinoma. In vitro function assays were conducted to explore growth and invasion of hepatocellular carcinoma cells. Next, the mechanism of hsa_circ_0006916 function in hepatocellular carcinoma was determined by luciferase reporter and RIP assays. Results: Hsa_circ_0006916 was substantially overexpressed in hepatocellular carci‑ noma tissues and cells. High levels of hsa_circ_0006916 in hepatocellular carcinoma patients were associated with advanced clinical characteristics. Down-regulation of hsa_circ_0006916 decreased the growth and invasion of hepatocellular carcinoma cells in vitro. The results suggested that hsa_circ_0006916 acted as a sponge of miR-337-3p and had an important functional use in the regulation of STAT3 levels in hepatocellular carcinoma cells. Moreover, miR-337-3p inhibition or STAT3 overexpression abolished the effect of hsa_circ_0006916 suppression on the progression of hepatocellular carci‑ noma cells. Conclusions: Our data suggest a novel hsa_circ_0006916/miR-337-3p/STAT3 axis in hepatocellular carcinoma, and provide a new target for treatment. Keywords: hsa_circ_0006916, miR-337-3p, STAT3, Hepatocellular carcinoma
Introduction Hepatocellular carcinoma is a common malignant disease worldwide with a high mortality rate [1, 2]. Although significant progress has been made in the field of hepatocellular carcinoma over the past decades, the prognosis remains inadequate because of a high recurrence and metastasis rate [3, 4]. Thus, it is crucial to determine the mechanism behind hepatocellular carcinoma tumorigenesis. Increasing data have shown that non-coding RNAs (ncRNAs) have a critical function in post-transcriptional gene regulation of different diseases [5, 6]. Circular RNA (circRNA) is a form of RNA composed of a covalent closed-loop structure, no 5′-3′ polarity, © The Author(s) 2020. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article
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