Hydrocarbon and Lipid Microbiology Protocols Biochemical Methods
This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and charact
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Hydrocarbon and Lipid Microbiology Protocols Biochemical Methods
Springer Protocols Handbooks
More information about this series at http://www.springer.com/series/8623
Terry J. McGenity
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Kenneth N. Timmis
Editors
Hydrocarbon and Lipid Microbiology Protocols Biochemical Methods Scientific Advisory Board Jack Gilbert, Ian Head, Mandy Joye, Victor de Lorenzo, Jan Roelof van der Meer, Colin Murrell, Josh Neufeld, Roger Prince, Juan Luis Ramos, Wilfred Ro¨ling, Heinz Wilkes, Michail Yakimov
•
Balbina Nogales
Editors Terry J. McGenity School of Biological Sciences University of Essex Colchester, Essex, UK
Kenneth N. Timmis Institute of Microbiology Technical University Braunschweig Braunschweig, Germany
Balbina Nogales Department of Biology University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC) Palma de Mallorca, Spain
ISSN 1949-2448 ISSN 1949-2456 (electronic) Springer Protocols Handbooks ISBN 978-3-662-49135-5 ISBN 978-3-662-49137-9 (eBook) DOI 10.1007/978-3-662-49137-9 Library of Congress Control Number: 2016938230 # Springer-Verlag Berlin Heidelberg 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer-Verlag GmbH Berlin Heidelberg
Preface to Hydrocarbon and Lipid Microbiology Protocols1
All active cellular systems require water as the principal medium and solvent for their metabolic and ecophysiological activities. Hydrophobic compounds and structures, which tend to exclude water, although providing inter alia excellent sources of energy and a means of biological compartmentalization, present problems of cellular handling, poor bioavailability and, in some cases, toxicity. Microbes both synthesize and exploit a vast range of hydrophobic organics, which includes biogenic lipids, oils and volatile compounds, geochemically transformed organics of biological origin (i.e. petroleum and other fossil hy
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