Hypertonic and Hypotonic Solutions

The colloidal behavior of protoplasm follows a queer pattern. One surprising and rather striking fact is that agents which might presumably be supposed to act in opposite fashion often enough have the same sort of an effect on protoplasm. So for example,

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L. V. HEILBR UNN

UND

F. WEBER GRAZ

PHILADELPHIA

MITHERAUSGEBER

w.

H. ARISZ-GRONINGEN . H. BAUER·WILHELMSHAVEN . J. BRACHET· BRUXELLES . H. G. CALLAN - ST. ANDREWS . R. COLLANDER- HELSINKI . K. DAN -TOKYO· E. FAURE-FREMIET-PARIS . A. FREY-WYSSLING-ZORICH' L. GEITLER-WIEN . K. HOFLER-WIEN . M. H. JACOBS-PHILADELPHIA· D. MAZIA-BERKELEY . A. MONROY-PALERMO . J. RUNNSTROM-STOCKHOLM . W. J. SCHMIDT - GIESSEN . S. STRUGGER -MONSTER

BAND II

CYTOPLASMA

c PHYSIK, PHYSIKALISCHE CHEMIE, KOLLOIDCHEMIE 1 THE VISCOSITY OF PROTOPLASM

WIEN

SPRINGER -VERLAG 1958

THE VISCOSITY OF PROTOPLASM BY

1. V. HEILBR UNN PHILADELPHIA WITH 23 FIGURES

WIEN SPRINGER·VERLAG 1958

ALLE RECHTE, INSBESONDERE DAS DER UBERSETZUNG IN FREMDE SPRACHEN, VORB~HALTEN. OHNE AUSDRUCKLICHE GENEHMIGUNG DES VERLAGES 1ST ES AUCH NICHT GESTATTET, DIESES BUCH ODER TEILE DARAUS AUF PHOTOMECHANISCHEM WEGE (PHOTOKOPIE, MIKROKOPIE) ZU VERVIELFALTIGEN. © BY SPRINGER·VERLAG IN VIENNA 1958.

ISBN-13: 978-3-211-80485-8 e-ISBN-13: 978-3-7091-5458-8 DOl: 10.1007/978-3-7091-5458-8

Protoplasmatologia II. Cytoplasm a C. Physik, Physikalische Chemie, Kolloidchemie 1. The Viscosity of Protoplasma

The Viscosity of Protoplasm By

L. V. HEILBRUNN Zoologlical Laboratory, University of Pennsylvania, Philadelphia, Pennsylvania With 23 Figures

Table of Contents Page

Introduction . . . . . . . . . . . . . . . . The Viscosity Concept . . . . . . . . . . . The Measurement of Protoplasmic Viscosity Absolute Viscosity . . . Plant Protoplasm . . Protozoan Protoplasm Muscle Protoplasm . . Nerve Protoplasm . . The Protoplasm of Marine Eggs A General Statement . . . . . The Effect of Various Physiological Factors The Action of Temperature . . . . . . The Action of Pressure and of Electricity Radiation Effects . . . . Visible Light Ultraviolet Radiation X Rays and Beta Rays The Action of Salts, Acids and Bases Hypertonic and Hypotonic Solutions Fat Solvents . . . . . . . . . . . . Cyanide and Azide . . . . . . Clotting and Anticlotting Substances Substances Used in the Treatment of Cancer Various Drugs . . . . . . . . . . . . Injury and Injury Substances . . . . The Significance of the Viscosity Data Bibliography . . . . . . . . . . Protoplasmatologia II, C, 1

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5 13 14 19

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48 50 50

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58 62 71

73 79 81

84 87 92 96 100

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II, C, 1: L. V.

HElL BRUNN,

The Viscosity of Protoplasm

Introduction From the very beginning of the study of protoplasm, biologists and cell physiologists have been concerned with the attempt to understand as much as possible about its physical state. But it is doubtful if in any other field of biology there has been more erroneous information gathered and perpetuated. This has in part been due to a lack of understanding of basic physical facts, in part also to the publication of numerous careless and untrustworthy observations. Microdissectionists prodded the cell with their microneedles, and they made many definite statements both concerning the relative and also the absolute viscosity of the protoplasm they attacked. These