Identification and comparative expression analysis of chalcone synthase, flavanone 3-hydroxylase and dihydroflavonol 4-r
- PDF / 1,657,522 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 29 Downloads / 196 Views
GENETICS & EVOLUTIONARY BIOLOGY - ORIGINAL ARTICLE
Identification and comparative expression analysis of chalcone synthase, flavanone 3‑hydroxylase and dihydroflavonol 4‑reductase genes in wild pomegranate (Punica granatum L.) organs Ramanjeet Kaur1 · Lubna Aslam1 · Nisha Kapoor1 · Ritu Mahajan1 Received: 10 April 2020 / Revised: 27 August 2020 / Accepted: 1 September 2020 / Published online: 16 September 2020 © Botanical Society of Sao Paulo 2020
Abstract Flavonoids are the predominant polyphenolic compounds which are ubiquitously present in plants, responsible for the colour development in different plant parts, thereby playing an important role in plant physiology. In order to understand chemodiversity and biological functions of flavonoids, it is important to understand flavonoid biosynthetic pathway that involves various structural genes. Three structural genes of flavonoid biosynthetic pathway, chalcone synthase (PgCHS; GenBank accession no: KY498075), flavanone 3-hydroxylase (PgF3H; GenBank accession no: MG012857) and dihydroflavonol 4-reductase (PgDFR; GenBank accession no: MG366127) were isolated and characterized from the wild Punica granatum. Molecular modelling indicated that the secondary structures of PgCHS, PgF3H and PgDFR are mainly α-helix and random coils. HPLC analysis of flavonoids showed its accumulation in different tissues and fruits, thus corroborating well with the expression profile of PgCHS, PgF3H and PgDFR genes. PgF3H and PgDFR genes exhibited high transcripts levels in red fruits while expression of Pg CHS gene was highest in flowers. Further, it is speculated that expression of all these structural genes is independent of each other. This is the first study that provides a comparative expression pattern of flavonoid genes playing a significant role in fruit ripening in wild pomegranate. Keywords Flavonoid biosynthesis · Gene expression · Phylogeny · Wild pomegranate
1 Introduction The development of fruit colour is attributed to the presence of flavonoids, produced in different organs in response to various developmental, environmental and genetic factors. Flavonoids are diverse group of secondary metabolites which occur ubiquitously in plants (Baskar et al. 2018). These are classified into various subgroups on the basis of their structural characteristics and on the nature of their C3 element (Castillo et al. 2018). These included flavonol, flavones, flavanone, flavan-3-ol, isoflavone and anthocyanin. Flavonoids play an important role in plant physiology. They Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40415-020-00648-x) contains supplementary material, which is available to authorized users. * Ritu Mahajan [email protected] 1
School of Biotechnology, University of Jammu, Jammu, Jammu & Kashmir, India
serves as major attractors for pollinators and dispensers, photoprotection from UV rays, pathogen attack and auxin transport (Taylor and Grotewold 2005; Mouradov and Spangenberg 2014). Each plant species has unique flavonoi
Data Loading...
