Identification and genome sequencing of RNA viruses in the eucalyptus snout beetle Gonipterus spp. (Coleoptera: Curculio
- PDF / 1,449,514 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 91 Downloads / 186 Views
ANNOTATED SEQUENCE RECORD
Identification and genome sequencing of RNA viruses in the eucalyptus snout beetle Gonipterus spp. (Coleoptera: Curculionidae) Leonardo A. Silva1 · Carolina Jordan2 · Vanessa Rafaela de Carvalho2 · Carlos F. Wilcken2 · Bergmann M. Ribeiro1 Received: 6 June 2020 / Accepted: 18 August 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract The genomes of two putative new RNA viruses (macula-like virus and bunya-like virus) were identified in total RNA extracted from dead eucalyptus snout beetles (Gonipterus spp.) from a laboratory colony. However, only bunya-like virus was detected in field-collected insects. The macula-like virus has a monopartite single-stranded RNA genome that contains three open reading frames (ORFs) encoding an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), protein with unknown function. The bunya-like virus genome was predicted to consist of two RNA segments: a large segment (L) encoding a single protein (RdRp) and a small segment (S) encoding a putative nucleocapsid protein. The genus Gonipterus (Coleoptera: Curculionidae) includes around 20 species of defoliating insects of Australian origin that are distributed worldwide. Two species of this genus were introduced in Brazil in the last century, Gonipterus platensis and G. pulverulentus. G. platensis, also known as eucalyptus snout beetle, has emerged as a major pest of Eucalyptus spp., causing important losses in wood production [1, 2]. Chemical insecticides have been widely used to control agricultural pests, but this method is costly and dangerous for non-target organisms in forestry. The use of biological control strategies to control insects reduces pollution by chemical pesticides and consequently benefits environment and human health. Currently, the biological control of G. platensis is carried out by Anaphes nitens (Girault) (Hymenoptera: Mymaridae), a tiny wasp that parasitizes
Handling Editor: Massimo Turina. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04814-0) contains supplementary material, which is available to authorized users. * Bergmann M. Ribeiro [email protected] 1
Laboratory of Baculovirus, Cell Biology Department, UnBUniversity of Brasília, 70910‑900 Brasília, DF, Brazil
Plant Protection Department, FCA/UNESP-São Paulo State University, Campus of Botucatu, 18610‑307 Botucatu, SP, Brazil
2
eggs of G. platensis, and by the entomopathogenic fungus Beauveria bassiana, but with limited effective control [2, 3]. There are few reports of viruses infecting beetles (Table S1). An RNA-sequencing approach using next-generation sequencing (NGS) was used to investigate the presence of viruses in dead insects from a colony of G. platensis maintained at the Biological Control of Forest Pests Laboratory, São Paulo State University (UNESP), Brazil. Ten dead insects (200 mg) were macerated in 1X phosphate-buffered saline (PBS-1X) (137.0 mM NaCl, 2.7 mM KCl, 10.0 mM Na2HPO4, 2.0 mM K H2PO4, pH 7.4). The ho
Data Loading...
