Identification of circular RNA hsa_circ_0044556 and its effect on the progression of colorectal cancer
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Cancer Cell International Open Access
PRIMARY RESEARCH
Identification of circular RNA hsa_ circ_0044556 and its effect on the progression of colorectal cancer Liang Jing, Junhui Wu, Xiaocheng Tang, Min Ma, Fei Long, Buning Tian* and Changwei Lin*
Abstract Background: Circular RNAs (circRNAs) are a novel class of noncoding RNAs. Increasing evidence indicates that circRNAs play an important role in the occurrence and development of tumors. However, the role of circRNA hsa_ circ_0044556 in the progression of colorectal cancer (CRC) remains unclear. Methods: First, we searched for differentially expressed circRNAs using a circRNA microarray in paired CRC and adjacent normal tissues. The circRNA hsa_circ_0044556 was screened out from the existing CRC circRNA microarray in the Gene Expression Omnibus database and our microarray. The clinical significance of hsa_circ_0044556 expression level in CRC patients was then investigated. Finally, the functions of the targets of this circRNA were determined in CRC cell lines. Results: Hsa_circ_0044556 was highly expressed in CRC patients and was positively correlated with tumor stage and lymph node metastasis. In CRC cell lines, the proliferation, migration, and invasion of cancer cells were inhibited by knocking down hsa_circ_0044556 expression. Conclusion: Hsa_circ_0044556 promoted the progression of CRC. It is possible that hsa_circ_0044556 will become a novel biomarker or therapeutic target for CRC. Keywords: Noncoding RNA, Circular RNA, Colorectal cancer, hsa_circ_0044556, Biomarker Background Colorectal cancer (CRC) is a malignant cancer that seriously endangers the health of humans. Currently, the global incidence rate of CRC ranks third among cancerrelated diseases with up to 1.2 million new cases each year, and more than 0.6 million deaths are expected each year [1]. From the perspective of the global incidence trend, the European and American regions are higher than the Asian and African regions, with the second place mortality rate (9.2%), and the developing countries are higher than the developed countries [2]. In 2015, China’s cancer statistics showed that the *Correspondence: [email protected]; [email protected] Department of Gastrointestinal Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China
incidence and the mortality of CRC ranked fifth among cancer-related diseases in China. The incidence of CRC and the death toll are rising yearly while the population with this disease trends younger [3]. Early diagnosis, accurate prognosis, and recurrence monitoring play important roles in cancer diagnosis and treatment. The 5-year survival rate of patients with advanced CRC is only 12%, while the 5-year survival rate of patients at the early stage can reach more than 90% [4]. Therefore, early diagnosis can significantly improve the prognosis as well as the survival and quality of life of patients with CRC. On this basis, the in-depth study of the pathophysiology mechanisms underlying the occurrence and development of CRC wil
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